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耳炎诺卡氏菌产生的含O-甲基葡萄糖脂多糖的分离与鉴定

Isolation and characterization of an O-methylglucose-containing lipopolysaccharide produced by Nocardia otitidis-caviarum.

作者信息

Pommier M T, Michel G

出版信息

J Gen Microbiol. 1986 Sep;132(9):2433-41. doi: 10.1099/00221287-132-9-2433.

DOI:10.1099/00221287-132-9-2433
PMID:3794652
Abstract

The low-Mr lipopolysaccharide produced by Nocardia otitidis-cavirum is composed of 6-O-methyl-D-glucose (11 mol), D-glucose (8 mol) and 3-O-methyl-D-glucose (1 mol). Glyceric acid was also found as a constituent. Methylation and periodate oxidation analyses suggested that the backbone was formed by (1----4)-linked glucose and O-methylglucose residues. Glucose (1 mol) and 6-O-methylglucose (1 mol) served as branching points. Acetic acid was the major acyl substituent esterifying glucose residues. The lipopolysaccharide was isolated from the cytoplasmic fraction. Several other Nocardia strains were examined; all possessed the same lipopolysaccharide in their extracts.

摘要

耳炎诺卡氏菌产生的低分子量脂多糖由6-O-甲基-D-葡萄糖(11摩尔)、D-葡萄糖(8摩尔)和3-O-甲基-D-葡萄糖(1摩尔)组成。还发现甘油酸是其成分之一。甲基化和高碘酸盐氧化分析表明,主链由(1→4)连接的葡萄糖和O-甲基葡萄糖残基形成。葡萄糖(1摩尔)和6-O-甲基葡萄糖(1摩尔)作为分支点。乙酸是酯化葡萄糖残基的主要酰基取代基。脂多糖是从细胞质部分分离出来的。对其他几种诺卡氏菌菌株进行了检测;所有菌株的提取物中都含有相同的脂多糖。

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Isolation and characterization of an O-methylglucose-containing lipopolysaccharide produced by Nocardia otitidis-caviarum.耳炎诺卡氏菌产生的含O-甲基葡萄糖脂多糖的分离与鉴定
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