Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, China.
Zhejinag Seed Industry Group Xinchuang Bio-breeding Co., Ltd., Hangzhou, China.
Pest Manag Sci. 2024 Jun;80(6):2515-2527. doi: 10.1002/ps.7878. Epub 2023 Dec 19.
The potential of insect viruses in the biological control of agricultural pests is well-recognized, yet their practical application faces obstacles such as host specificity, variable virulence, and resource scarcity. High-throughput sequencing (HTS) technologies have significantly advanced our capabilities in discovering and identifying new insect viruses, thereby enriching the arsenal for pest management. Concurrently, progress in reverse genetics has facilitated the development of versatile viral expression vectors. These vectors have enhanced the specificity and effectiveness of insect viruses in targeting specific pests, offering a more precise approach to pest control. This review provides a comprehensive examination of the methodologies employed in the identification of insect viruses using HTS. Additionally, it explores the domain of genetically modified insect viruses and their associated challenges in pest management. The adoption of these cutting-edge approaches holds great promise for developing environmentally sustainable and effective pest control solutions. © 2023 Society of Chemical Industry.
昆虫病毒在农业害虫生物防治中的潜力已得到广泛认可，但在实际应用中仍面临宿主特异性、毒力变化和资源匮乏等障碍。高通量测序（HTS）技术极大地提高了我们发现和鉴定新昆虫病毒的能力，从而丰富了害虫管理的手段。与此同时，反向遗传学的进展促进了多功能病毒表达载体的发展。这些载体提高了昆虫病毒针对特定害虫的特异性和有效性，为害虫防治提供了更精确的方法。本综述全面考察了使用 HTS 鉴定昆虫病毒的方法。此外，还探讨了遗传修饰昆虫病毒的领域及其在害虫管理中面临的挑战。采用这些前沿方法有望开发出环境可持续且有效的害虫防治解决方案。© 2023 英国化学学会。
