静脉给予抗坏血酸（维生素 C）对健康成年马氧化状态的影响。

Effects of intravenous administration of ascorbic acid (vitamin C) on oxidative status in healthy adult horses.

机构信息

Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Purdue University, West Lafayette, Indiana, USA.

Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens, Georgia, USA.

出版信息

J Vet Intern Med. 2024 Jan-Feb;38(1):460-468. doi: 10.1111/jvim.16934. Epub 2023 Nov 10.

DOI:10.1111/jvim.16934

PMID:37948618

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10800205/

Abstract

BACKGROUND

Ascorbic acid (AA) is an antioxidant that might be beneficial for adjunctive treatment of sepsis in horses. The optimal dose and effects on oxidative status are unknown.

HYPOTHESIS

Ascorbic acid administration will increase plasma AA concentrations and decrease determinants of reactive oxygen metabolites (dROM), basal and stimulant-induced intraerythrocytic reactive oxygen species (ROS) concentrations, and stimulant-induced neutrophil ROS production, and increase plasma antioxidant capacity (PAC) in a dose-dependent manner.

ANIMALS

Eight healthy horses.

METHODS

Randomized placebo-controlled crossover study. Each horse received 4 single-dose IV treatments including AA at 25, 50, and 100 mg/kg and saline (placebo) with each treatment separated by ≥1 week. Blood was collected at baseline, 2 and 6 hours for assessment of plasma dROM and PAC via photometer, intraerythrocytic ROS by flow cytometry, and stimulant-induced neutrophil ROS by a fluorometric assay. Plasma AA concentrations were measured by high-performance liquid chromatography/electrochemical detection.

RESULTS

Ascorbic acid at 100 mg/kg resulted in decreased dROM 2 hours after treatment (P = .03, 95% CI 5.51-121.2, point estimate 63.3). There was no effect of AA on basal or stimulant-induced intraerythrocytic ROS (P = .88, 95% CI -0.156 to 0.081, point estimate -0.037; P = .93, 95% CI -0.123 to 0.112, point estimate -0.006, respectively), basal or stimulant-induced neutrophil ROS (P ≥ .12, 95% CI -644.9 to 56.2, point estimate -294.4), or PAC (P ≥ .64, 95% CI -1567 to 463.4, point estimate -552.0) at any dose or timepoint. Plasma AA concentrations increased in a dose-dependent manner.

CONCLUSIONS AND CLINICAL IMPORTANCE

High-dose administration of AA might provide antioxidant benefits in horses.

摘要

背景

抗坏血酸（AA）是一种抗氧化剂，可能有益于马败血症的辅助治疗。最佳剂量和对氧化状态的影响尚不清楚。

假设

抗坏血酸给药将增加血浆 AA 浓度，并降低反应性氧代谢物（dROM）、基础和刺激诱导的红细胞内活性氧（ROS）浓度以及刺激诱导的中性粒细胞 ROS 产生的决定因素，并且以剂量依赖性方式增加血浆抗氧化能力（PAC）。

动物

8 匹健康马。

方法

随机安慰剂对照交叉研究。每匹马接受 4 次单剂量 IV 治疗，包括 25、50 和 100mg/kg 的 AA 和盐水（安慰剂），每种治疗方法之间至少间隔 1 周。在基线、2 小时和 6 小时采集血液，通过光度计评估血浆 dROM 和 PAC，通过流式细胞术评估红细胞内 ROS，通过荧光测定法评估刺激诱导的中性粒细胞 ROS。通过高效液相色谱/电化学检测测量血浆 AA 浓度。

结果

100mg/kg 的 AA 治疗后 2 小时降低了 dROM（P = .03，95%CI 5.51-121.2，点估计 63.3）。AA 对基础或刺激诱导的红细胞内 ROS 没有影响（P = .88，95%CI -0.156 至 0.081，点估计 -0.037；P = .93，95%CI -0.123 至 0.112，点估计 -0.006，分别），基础或刺激诱导的中性粒细胞 ROS（P ≥ .12，95%CI -644.9 至 56.2，点估计 -294.4）或 PAC（P ≥ .64，95%CI -1567 至 463.4，点估计 -552.0）在任何剂量或时间点均无影响。血浆 AA 浓度呈剂量依赖性增加。