Cable B L, John D T
J Protozool. 1986 Nov;33(4):467-72. doi: 10.1111/j.1550-7408.1986.tb05643.x.
Ameba to flagellate transformation in Naegleria fowleri (Lovell strain) was affected by growth temperature, phase of growth, strain of ameba, culture agitation, enflagellation temperature, enflagellation diluent, and cell concentration. Amebae transformed best when they were grown without agitation and enflagellated with agitation. Regardless of growth temperature (23 degrees, 30 degrees, 37 degrees, and 42 degrees C were tested), amebae transformed best at 37 degrees C. Enflagellation was greatest for cells harvested between 24 h (mid-exponential) and 84 h (late stationary) of growth.
福氏耐格里阿米巴(洛弗尔菌株)中阿米巴向鞭毛虫的转化受生长温度、生长阶段、阿米巴菌株、培养振荡、成鞭毛温度、成鞭毛稀释液和细胞浓度的影响。阿米巴在无振荡条件下生长并在振荡条件下成鞭毛时转化效果最佳。无论生长温度如何(测试了23℃、30℃、37℃和42℃),阿米巴在37℃时转化效果最佳。对于在生长24小时(指数中期)至84小时(稳定后期)之间收获的细胞,成鞭毛情况最为显著。