Cao Wenhai, Meng Xiaotong, Cao Fangming, Wang Jinpeng, Yang Maowei
Department of Orthopedics, First Hospital of China Medical University, Shenyang, China.
iScience. 2023 Oct 17;26(11):108236. doi: 10.1016/j.isci.2023.108236. eCollection 2023 Nov 17.
Diabetic non-healing wounds are bringing a heavy burden on patients and society. Platelet-rich plasma (PRP) has been widely applied in tissue regenerating for containing various growth factors. Recently, PRP-derived exosomes (PRP-Exos) have been proved to be more effective than PRP in tissue regeneration. However, few studies have investigated the therapeutic potential of PRP-Exos in diabetic wound healing to date. Therefore, we extracted and identified exosomes derived from PRP and tested its promoting effect on diabetic wound healing and . We found that high glucose (HG) inhibited cell proliferation and migration and induced apoptosis through ROS-dependent activation of the JNK and p38 MAPK signaling pathways. PRP-Exos can stimulate fibroblast functions and accelerate diabetic wound healing. The benefits of PRP-Exos may be attributed to its capability to prevent HG-induced ROS-dependent apoptosis via the PDGF-BB/JAK2/STAT3/Bcl-2 signaling pathway. This illustrates the therapeutic potential of PRP-Exos in diabetic wounds.
糖尿病难愈性伤口给患者和社会带来了沉重负担。富含血小板血浆（PRP）因含有多种生长因子而被广泛应用于组织再生。近来，PRP衍生的外泌体（PRP-Exos）已被证明在组织再生方面比PRP更有效。然而，迄今为止，很少有研究探讨PRP-Exos在糖尿病伤口愈合中的治疗潜力。因此，我们提取并鉴定了PRP衍生的外泌体，并测试了其对糖尿病伤口愈合的促进作用。我们发现高糖（HG）通过ROS依赖的JNK和p38 MAPK信号通路激活抑制细胞增殖和迁移并诱导细胞凋亡。PRP-Exos可刺激成纤维细胞功能并加速糖尿病伤口愈合。PRP-Exos的益处可能归因于其通过PDGF-BB/JAK2/STAT3/Bcl-2信号通路预防HG诱导的ROS依赖的细胞凋亡的能力。这说明了PRP-Exos在糖尿病伤口中的治疗潜力。
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