Hussein Kajeen, Salih Sherwan, Al-Timimi Dhia
Department of Medical Chemistry, College of Medicine, University of Duhok, Duhok, IRQ.
Cureus. 2023 Oct 11;15(10):e46829. doi: 10.7759/cureus.46829. eCollection 2023 Oct.
Background Polymorphisms in the () and genes have been linked to severe hypertriglyceridemia in several populations. This study investigated the frequency of Hind lllandAva ll polymorphism among Kurdish patients with severe hypertriglyceridemia. Methodology We investigated Hind llland -Ava ll gene polymorphism in a sample of Kurdish patients receiving treatment at Azadi Teaching Hospital in Duhok, Kurdistan Region, Iraq. We included a total of 100 subjects in this study, of which 64 had severe hypertriglyceridemia, and 36 had normotriglyceridemia. There were 56 males and 44 females. We used the polymerase chain reaction-restriction fragment length polymorphism technique to determine the polymorphism of the Hind lll and Ava ll genes. Results In those with severe hypertriglyceridemia, the most frequent alleles were H+H+ Hind lll polymorphism (42, 65.6%) followed by A1A1 Ava ll polymorphism (30, 46.9%), whereas these frequencies were 16 (44.4%) and 6 (16.7%) in those with normotriglyceridemia, respectively. The H+H+ genotype group had considerably higher triglyceride levels and lower high-density lipoprotein cholesterol levels compared with the H-H- genotype group. A similar pattern was observed when comparing the A1A1 and A2A2 genotype groups, with both patterns being statistically significant. Conclusions Our results showed a high frequency of H+H+ Hind III polymorphism in those with hypertriglyceridemia, which may be a hereditary indicator of vulnerability to this condition in the Kurdish population.
背景 在多个群体中，（）基因和基因的多态性与严重高甘油三酯血症有关。本研究调查了库尔德族严重高甘油三酯血症患者中Hind III和Ava II多态性的频率。方法 我们在伊拉克库尔德地区杜胡克的阿扎迪教学医院，对接受治疗的库尔德族患者样本进行了Hind III和Ava II基因多态性调查。本研究共纳入100名受试者，其中64人患有严重高甘油三酯血症，36人甘油三酯水平正常。有56名男性和44名女性。我们使用聚合酶链反应-限制性片段长度多态性技术来确定Hind III和Ava II基因的多态性。结果 在严重高甘油三酯血症患者中，最常见的等位基因是H+H+ Hind III多态性（42例，65.6%），其次是A1A1 Ava II多态性（30例，46.9%），而在甘油三酯水平正常的患者中，这些频率分别为16例（44.4%）和6例（16.7%）。与H-H-基因型组相比，H+H+基因型组的甘油三酯水平显著更高，高密度脂蛋白胆固醇水平更低。比较A1A1和A2A2基因型组时也观察到类似模式，两种模式均具有统计学意义。结论 我们的结果显示，高甘油三酯血症患者中H+H+ Hind III多态性的频率较高，这可能是库尔德人群中易患此病的遗传指标。
