Department of Oncology, Jinan Central Hospital, Shandong University, Jinan, China.
Department of Thoracic Surgery, Jinan Central Hospital, Shandong University, Jinan, China.
Pol J Pathol. 2023;74(3):194-202. doi: 10.5114/pjp.2023.132224.
Cutaneous carcinoma is one of the most common neoplasm tumors in the West. Its incidence rate is one of the fastest growing tumors in China. The Krüppel-like factor 6 (KLF6) is a latent tumor suppressor. Decreased KLF6 is related to the occurrence and progression of many cancers in human. Our previous studies have demonstrated that KLF6 was down-regulation in cutaneous malignant melanoma (CMM), and was significant correlated with ulcer, lymph node metastasis and clinical stage, suggesting that KLF6 loss is expected to become a biological indicator of poor prognosis in CMM patients. In this research, we would further study the features of KLF6 in the malignant progression of CMM. The expression of KLF6 was up-regulated by lentivirus infection containing KLF6, and short hairpin RNA (shRNA) was used for knockdown of KLF6 in CMM cells. Western blot, RT-qpcr, CCK8 assay, transwell migration assays, wound healing assay and flow cytometry were used to test the role of KLF6 in the CMM. We found that reduced expression of KLF6 significantly enhanced proliferation, migration and invasion. Moreover, KLF6 induced CMM cell apoptosis and G1 cycle arrest. The decreased KLF6 expression is expected to be a biological indicator of poor prognosis in CMM patients.
皮肤癌是西方最常见的实体肿瘤之一。在中国，它是发病率增长最快的肿瘤之一。 Krüppel 样因子 6（KLF6）是一种潜在的肿瘤抑制因子。KLF6 的减少与人类许多癌症的发生和发展有关。我们之前的研究表明，KLF6 在皮肤恶性黑色素瘤（CMM）中下调，并且与溃疡、淋巴结转移和临床分期显著相关，表明 KLF6 的缺失有望成为 CMM 患者预后不良的生物学指标。在这项研究中，我们将进一步研究 KLF6 在 CMM 恶性进展中的特征。通过慢病毒感染含有 KLF6 的病毒来上调 KLF6 的表达，并使用短发夹 RNA（shRNA）敲低 CMM 细胞中的 KLF6。Western blot、RT-qpcr、CCK8 测定、Transwell 迁移测定、划痕愈合测定和流式细胞术用于测试 KLF6 在 CMM 中的作用。我们发现，KLF6 的表达减少显著增强了增殖、迁移和侵袭。此外，KLF6 诱导 CMM 细胞凋亡和 G1 周期阻滞。KLF6 的减少表达有望成为 CMM 患者预后不良的生物学指标。
