College of Life Sciences, National Engineering Research Center for Efficient Utilization of Soil and Fertilizer Resources, Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-alkali Land, Shandong Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai'an 271018, China; Shandong Baolai-leelai Bioengineering Co., Ltd., Tai'an 271000, China.
State Key Laboratory of Biobased Material and Green Papermaking, School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), and The State Key Laboratory of Microbial Technology, Jinan 250353, China.
Bioresour Technol. 2024 Jan;392:130002. doi: 10.1016/j.biortech.2023.130002. Epub 2023 Nov 11.
2,3-Butanediol is an essential renewable fuel. The synthesis of 2,3-butanediol using Paenibacillus polymyxa has attracted increasing attention. In this study, the glucose-derived 2,3-butanediol pathway and its related genes were identified in P. polymyxa using combined transcriptome and metabolome analyses. The functions of two distinct genes ldh1 and ldh3 encoding lactate dehydrogenase, the gene bdh encoding butanediol dehydrogenase, and the spore-forming genes spo0A and spoIIE were studied and directly knocked out or overexpressed in the genome sequence to improve the production of 2,3-butanediol. A raw hydrolysate of poplar wood containing 27 g/L glucose and 15 g/L xylose was used to produce 2,3-butanediol with a maximum yield of 0.465 g/g and 93 % of the maximum theoretical value, and the total production of 2,3-butanediol and ethanol reached 21.7 g/L. This study provides a new scheme for engineered P. polymyxa to produce renewable fuels using raw poplar wood hydrolysates.
2,3-丁二醇是一种必不可少的可再生燃料。利用多粘类芽孢杆菌合成 2,3-丁二醇引起了越来越多的关注。在这项研究中，通过组合转录组和代谢组分析，鉴定出多粘类芽孢杆菌中的葡萄糖衍生的 2,3-丁二醇途径及其相关基因。研究了编码乳酸脱氢酶的两个不同基因 ldh1 和 ldh3、编码丁二醇脱氢酶的基因 bdh 以及产孢基因 spo0A 和 spoIIE 的功能，并在基因组序列中直接敲除或过表达这些基因，以提高 2,3-丁二醇的产量。利用含有 27 g/L 葡萄糖和 15 g/L 木糖的杨木原料水解液生产 2,3-丁二醇，最大产量为 0.465 g/g，达到最大理论值的 93%，2,3-丁二醇和乙醇的总产量达到 21.7 g/L。本研究为利用杨木原料水解液生产可再生燃料的工程化多粘类芽孢杆菌提供了一种新方案。
