• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

嵴动力学在生物能量受损的线粒体中受到调节。

Cristae dynamics is modulated in bioenergetically compromised mitochondria.

机构信息

https://ror.org/024z2rq82 Institute of Biochemistry and Molecular Biology I, Medical Faculty and University Hospital Düsseldorf, Heinrich-Heine-University Düsseldorf, Düsseldorf, Germany.

https://ror.org/024z2rq82 Center for Advanced Imaging, Faculty of Mathematics and Natural Sciences, Heinrich Heine University Düsseldorf, Düsseldorf, Germany.

出版信息

Life Sci Alliance. 2023 Nov 13;7(2). doi: 10.26508/lsa.202302386. Print 2024 Feb.

DOI:10.26508/lsa.202302386
PMID:37957016
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10643176/
Abstract

Cristae membranes have been recently shown to undergo intramitochondrial merging and splitting events. Yet, the metabolic and bioenergetic factors regulating them are unclear. Here, we investigated whether and how cristae morphology and dynamics are dependent on oxidative phosphorylation (OXPHOS) complexes, the mitochondrial membrane potential (ΔΨ), and the ADP/ATP nucleotide translocator. Advanced live-cell STED nanoscopy combined with in-depth quantification were employed to analyse cristae morphology and dynamics after treatment of mammalian cells with rotenone, antimycin A, oligomycin A, and CCCP. This led to formation of enlarged mitochondria along with reduced cristae density but did not impair cristae dynamics. CCCP treatment leading to ΔΨ abrogation even enhanced cristae dynamics showing its ΔΨ-independent nature. Inhibition of OXPHOS complexes was accompanied by reduced ATP levels but did not affect cristae dynamics. However, inhibition of ADP/ATP exchange led to aberrant cristae morphology and impaired cristae dynamics in a mitochondrial subset. In sum, we provide quantitative data of cristae membrane remodelling under different conditions supporting an important interplay between OXPHOS, metabolite exchange, and cristae membrane dynamics.

摘要

嵴膜最近被证明会发生线粒体内部融合和分裂事件。然而，调节它们的代谢和生物能量因素尚不清楚。在这里，我们研究了嵴的形态和动力学是否以及如何依赖于氧化磷酸化（OXPHOS）复合物、线粒体膜电位（ΔΨ）和 ADP/ATP 核苷酸转位酶。先进的活细胞 STED 纳米显微镜结合深入的定量分析，用于分析哺乳动物细胞用鱼藤酮、抗霉素 A、寡霉素 A 和 CCCP 处理后嵴的形态和动力学。这导致形成了增大的线粒体，同时嵴密度降低，但不损害嵴的动力学。导致 ΔΨ 消除的 CCCP 处理甚至增强了嵴的动力学，表明其具有 ΔΨ 独立性。OXPHOS 复合物的抑制伴随着 ATP 水平的降低，但不影响嵴的动力学。然而，ADP/ATP 交换的抑制导致线粒体亚群中嵴的形态异常和嵴动力学受损。总之，我们提供了不同条件下嵴膜重塑的定量数据，支持 OXPHOS、代谢物交换和嵴膜动力学之间的重要相互作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/a8572cd6d60a/LSA-2023-02386_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/9453db4d3eb1/LSA-2023-02386_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/969df16a402e/LSA-2023-02386_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/ebeb4b91d058/LSA-2023-02386_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/e3aa7e74b510/LSA-2023-02386_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/e947b95925e8/LSA-2023-02386_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/037fe7d3dff7/LSA-2023-02386_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/87ecb59fd1cd/LSA-2023-02386_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/b4aa6a08e53a/LSA-2023-02386_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/fffa3fff90cb/LSA-2023-02386_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/deb080703728/LSA-2023-02386_FigS6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/0f40d78e72e2/LSA-2023-02386_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/39551c31df37/LSA-2023-02386_FigS7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/a8572cd6d60a/LSA-2023-02386_Fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/9453db4d3eb1/LSA-2023-02386_FigS1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/969df16a402e/LSA-2023-02386_Fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/ebeb4b91d058/LSA-2023-02386_FigS2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/e3aa7e74b510/LSA-2023-02386_Fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/e947b95925e8/LSA-2023-02386_FigS3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/037fe7d3dff7/LSA-2023-02386_Fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/87ecb59fd1cd/LSA-2023-02386_FigS4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/b4aa6a08e53a/LSA-2023-02386_FigS5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/fffa3fff90cb/LSA-2023-02386_Fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/deb080703728/LSA-2023-02386_FigS6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/0f40d78e72e2/LSA-2023-02386_Fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/39551c31df37/LSA-2023-02386_FigS7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5722/10643176/a8572cd6d60a/LSA-2023-02386_Fig6.jpg

相似文献

1
Cristae dynamics is modulated in bioenergetically compromised mitochondria.嵴动力学在生物能量受损的线粒体中受到调节。
Life Sci Alliance. 2023 Nov 13;7(2). doi: 10.26508/lsa.202302386. Print 2024 Feb.
2
Individual cristae within the same mitochondrion display different membrane potentials and are functionally independent.同一线粒体中的个体嵴显示出不同的膜电位，并且在功能上是独立的。
EMBO J. 2019 Nov 15;38(22):e101056. doi: 10.15252/embj.2018101056. Epub 2019 Oct 14.
3
Effects of proton pumping on the structural rigidity of cristae in mitochondria.质子泵对线粒体嵴结构刚性的影响。
Arch Biochem Biophys. 2022 May 15;720:109172. doi: 10.1016/j.abb.2022.109172. Epub 2022 Mar 8.
4
Calcium sequestering ability of mitochondria modulates influx of calcium through glutamate receptor channel.线粒体的钙螯合能力通过谷氨酸受体通道调节钙的流入。
Neurochem Res. 2000 Dec;25(12):1527-36. doi: 10.1023/a:1026602100160.
5
Supercomplex supercomplexes: Raison d'etre and functional significance of supramolecular organization in oxidative phosphorylation.超级复合物超级复合物：氧化磷酸化中超分子组织的存在理由和功能意义。
Biomol Concepts. 2022 May 26;13(1):272-288. doi: 10.1515/bmc-2022-0021.
6
Mitochondrial cristae in health and disease.健康与疾病状态下的线粒体嵴
Int J Biol Macromol. 2023 Apr 30;235:123755. doi: 10.1016/j.ijbiomac.2023.123755. Epub 2023 Feb 20.
7
Who and how in the regulation of mitochondrial cristae shape and function.谁和如何调节线粒体嵴的形态和功能。
Biochem Biophys Res Commun. 2018 May 27;500(1):94-101. doi: 10.1016/j.bbrc.2017.04.088. Epub 2017 Apr 21.
8
Age-related changes in the mitochondria of human mural granulosa cells.人壁颗粒细胞中线粒体的年龄相关性变化。
Hum Reprod. 2017 Dec 1;32(12):2465-2473. doi: 10.1093/humrep/dex309.
9
Cristae undergo continuous cycles of membrane remodelling in a MICOS-dependent manner.嵴通过 MICOS 依赖性的连续膜重塑循环。
EMBO Rep. 2020 Mar 4;21(3):e49776. doi: 10.15252/embr.201949776. Epub 2020 Feb 18.
10
Inner mitochondrial membrane compartmentalization: Dynamics across scales.线粒体内部膜区室的分隔：跨越多个尺度的动力学。
Int J Biochem Cell Biol. 2020 Mar;120:105694. doi: 10.1016/j.biocel.2020.105694. Epub 2020 Jan 10.

引用本文的文献

1
Molecular machineries shaping the mitochondrial inner membrane.塑造线粒体内膜的分子机制。
Nat Rev Mol Cell Biol. 2025 May 14. doi: 10.1038/s41580-025-00854-z.
2
How the Topology of the Mitochondrial Inner Membrane Modulates ATP Production.线粒体内膜的拓扑结构如何调节ATP的产生。
Cells. 2025 Feb 11;14(4):257. doi: 10.3390/cells14040257.
3
The Pivotal Role of LACTB in the Process of Cancer Development.LACTB在癌症发展过程中的关键作用。

本文引用的文献

1
Intracytoplasmic-membrane development in alphaproteobacteria involves the homolog of the mitochondrial crista-developing protein Mic60.α-变形菌纲细菌的胞内膜发育涉及线粒体嵴发育蛋白Mic60的同源物。
Curr Biol. 2023 Mar 27;33(6):1099-1111.e6. doi: 10.1016/j.cub.2023.02.059. Epub 2023 Mar 14.
2
Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain.多色活细胞 STED 纳米显微镜观察具有温和内膜染色的线粒体。
Proc Natl Acad Sci U S A. 2022 Dec 27;119(52):e2215799119. doi: 10.1073/pnas.2215799119. Epub 2022 Dec 19.
3
Mitochondrial developmental encephalopathy with bilateral optic neuropathy related to homozygous variants in IMMT gene.
Int J Mol Sci. 2025 Feb 1;26(3):1279. doi: 10.3390/ijms26031279.
4
Molecular Profiling of Mouse Models of Loss or Gain of Function of the KCNT1 (Slack) Potassium Channel and Antisense Oligonucleotide Treatment.KCNT1（松弛）钾通道功能丧失或获得的小鼠模型的分子谱分析及反义寡核苷酸治疗。
Biomolecules. 2024 Nov 2;14(11):1397. doi: 10.3390/biom14111397.
5
Warm Cells, Hot Mitochondria: Achievements and Problems of Ultralocal Thermometry.暖细胞，热线粒体：超局域测温的成就与问题。
Int J Mol Sci. 2023 Nov 29;24(23):16955. doi: 10.3390/ijms242316955.
线粒体发育性脑病伴双侧视神经病变与 IMMT 基因纯合变异相关。
Clin Genet. 2022 Feb;101(2):233-241. doi: 10.1111/cge.14093. Epub 2021 Dec 5.
4
Reversible and irreversible mitochondrial swelling: Effects of variable mitochondrial activity.可逆性和不可逆性线粒体肿胀：线粒体活性变化的影响。
Biosystems. 2021 Dec;210:104559. doi: 10.1016/j.biosystems.2021.104559. Epub 2021 Oct 7.
5
Reversible and irreversible mitochondrial swelling in vitro.体外可逆性和不可逆性线粒体肿胀
Biophys Chem. 2021 Nov;278:106668. doi: 10.1016/j.bpc.2021.106668. Epub 2021 Aug 14.
6
Conserved GxxxG and WN motifs of MIC13 are essential for bridging two MICOS subcomplexes.MIC13 保守的 GxxxG 和 WN 基序对于桥接两个 MICOS 亚基复合物是必需的。
Biochim Biophys Acta Biomembr. 2021 Dec 1;1863(12):183683. doi: 10.1016/j.bbamem.2021.183683. Epub 2021 Jul 13.
7
Emerging Roles of the MICOS Complex in Cristae Dynamics and Biogenesis.MICOS复合体在嵴动力学和生物发生中的新作用。
Biology (Basel). 2021 Jun 29;10(7):600. doi: 10.3390/biology10070600.
8
OPA1 and MICOS Regulate mitochondrial crista dynamics and formation.OPA1 和 MICOS 调节线粒体嵴的动态和形成。
Cell Death Dis. 2020 Oct 31;11(10):940. doi: 10.1038/s41419-020-03152-y.
9
Cristae Membrane Dynamics - A Paradigm Change.嵴膜动力学——范式转变。
Trends Cell Biol. 2020 Dec;30(12):923-936. doi: 10.1016/j.tcb.2020.08.008. Epub 2020 Sep 23.
10
MICOS assembly controls mitochondrial inner membrane remodeling and crista junction redistribution to mediate cristae formation.MICOS 组装控制线粒体内膜重塑和嵴连接再分布，以介导嵴的形成。
EMBO J. 2020 Jul 15;39(14):e104105. doi: 10.15252/embj.2019104105. Epub 2020 Jun 22.