Rozovskaia T A, Bibilashvili P Sh, Tarusova N B, Gurskiĭ G V, Strel'tsov S A
Mol Biol (Mosk). 1977 May-Jun;11(3):598-610.
3'(2')-O-acyl derivatives of the uridine triphosphate were synthesized. Acyl residues contained fluorescent dye; fluoresceine or rodamine C. Optical properties and stability of UTP analogues were studied. Their ability to serve as the substrates for calf thymus terminal deoxyribonucleotidyl transferase and E. coli RNA polymerase was also examined. It was shown that both enzymes were able to use tested analogues as substrates. Incorporation of the analogues into nascent RNA and DNA chains inhibited the synthetic reaction because of primer inactivation. The rate of the incorporation of the analogues showed an exponential time dependence
合成了尿苷三磷酸的3'(2')-O-酰基衍生物。酰基残基含有荧光染料；荧光素或罗丹明C。研究了尿苷三磷酸类似物的光学性质和稳定性。还检测了它们作为小牛胸腺末端脱氧核糖核苷酸转移酶和大肠杆菌RNA聚合酶底物的能力。结果表明，这两种酶都能够将测试的类似物用作底物。由于引物失活，类似物掺入新生RNA和DNA链中会抑制合成反应。类似物的掺入速率呈指数时间依赖性
