Polonskiĭ Iu S, Naĭdenova N M, Kozlov Iu I, Zograf Iu N, Debabov V G
Mol Biol (Mosk). 1977 May-Jun;11(3):656-60.
Influence of the protein product of F1 phage gene 5 (protein 5) on the synthesis RNA and poly(A) in vitro was studied. It has been shown, that protein 5 has no effect on the transcription of the native DNA by E. coli RNA-polymerase, but completely prevents RNA and poly(A) synthesis on the denatured or single-stranded DNA at the protein/DNA ratio 10:1. Protein 5 inhibits poly(A) synthesis with oligo(dT)9 and oligo(dT)12 as a template, preventing binding of enzyme to the oligonucleotide. After the initiation of the poly(A) synthesis the inhibition becomes considerably wearer. The biological function of the inhibition of the transcription by "unwinding" proteins is discussed.
研究了F1噬菌体基因5的蛋白质产物（蛋白质5）对体外RNA和多聚腺苷酸（poly(A)）合成的影响。结果表明，蛋白质5对大肠杆菌RNA聚合酶转录天然DNA没有影响，但在蛋白质/DNA比例为10:1时，能完全阻止变性或单链DNA上的RNA和多聚腺苷酸合成。蛋白质5以寡聚（dT）9和寡聚（dT）12为模板抑制多聚腺苷酸合成，阻止酶与寡核苷酸结合。多聚腺苷酸合成起始后，抑制作用明显减弱。文中讨论了“解旋”蛋白抑制转录的生物学功能。
