环状 RNA CircUBAP2 通过调控前列腺癌中的 miR-143/TFAP2B 轴促进肿瘤进展。
Novel Circular RNA CircUBAP2 Drives Tumor Progression by Regulating the miR-143/TFAP2B Axis in Prostate Cancer.
机构信息
Department of Urology Surgery, Wujin Hospital Affiliated with Jiangsu University, the Wujin Clinical College of Xuzhou Medical University, Changzhou, Jiangsu 213017, China.
出版信息
Protein Pept Lett. 2024;31(1):61-73. doi: 10.2174/0109298665268943231103114654.
BACKGROUND
More and more investigations reveal that circular RNAs (circRNAs) are involved in cancer progression. CircRNA UBAP2 was closely related to prostate cancer. However, the biological function and specifical mechanism of circUBAP2 are still poorly discovered in prostate cancer (PCa).
OBJECTIVES
This study aims to explore the biological function and mechanism of circUBAP2 in PCa.
METHODS
The levels of mRNA and proteins were assessed by qRT-PCR assay and Western blot, respectively. Cell growth, migration, and invasion ability were measured using CCK-8 assay and Transwell assay. Apoptosis was assessed using flow cytometry. The interactions between circUBAP2, miR-143, and TFAP2B were determined by luciferase report assay. The tumor growth was determined by in vivo tumor formation assay. The tumor morphology was assessed using H&E staining assay, and immunohistochemistry assay was conducted to assess the level of KI67.
RESULTS
We found circUBAP2 and TFAP2B were notably elevated, while miR-143 was largely attenuated in prostate cancer cells and tissues. CircUBAP2 was found to affect cell viability, metastasis and EMT, while attenuating the apoptosis rate of prostate cancer cells. CircUBAP2 directly targeted miR-143, and miR-143 inhibitor could reverse the effects that circUBAP2 interference-induced in prostate cancer cells. TFAP2B is directly bound to miR-143, and overexpression of TFAP2B could attenuate the influences that miR-143-induced in prostate cancer cells.
CONCLUSION
CircUBAP2 promoted prostate cancer progression via miR-143/TFAP2B axis.
背景
越来越多的研究表明，环状 RNA（circRNAs）参与了癌症的进展。环状 RNA UBAP2 与前列腺癌密切相关。然而，circUBAP2 在前列腺癌（PCa）中的生物学功能和具体机制仍知之甚少。
目的
本研究旨在探讨 circUBAP2 在 PCa 中的生物学功能和机制。
方法
通过 qRT-PCR 检测和 Western blot 分别评估 mRNA 和蛋白质水平。使用 CCK-8 检测和 Transwell 检测分别评估细胞生长、迁移和侵袭能力。使用流式细胞术评估细胞凋亡。通过荧光素酶报告实验确定 circUBAP2、miR-143 和 TFAP2B 之间的相互作用。通过体内肿瘤形成实验确定肿瘤生长。使用 H&E 染色实验评估肿瘤形态，使用免疫组织化学实验评估 KI67 水平。
结果
我们发现 circUBAP2 和 TFAP2B 在前列腺癌细胞和组织中显著上调，而 miR-143 则显著下调。circUBAP2 被发现影响细胞活力、转移和 EMT，同时降低前列腺癌细胞的凋亡率。circUBAP2 可直接靶向 miR-143，而 miR-143 抑制剂可逆转 circUBAP2 干扰引起的前列腺癌细胞的作用。TFAP2B 可直接与 miR-143 结合，过表达 TFAP2B 可减弱 miR-143 引起的前列腺癌细胞的影响。
结论
circUBAP2 通过 miR-143/TFAP2B 轴促进前列腺癌的进展。
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