Jiang Wen-Jie, Lee Song-Hee, Heo Geun, Chung Hak Jae, Cho Eun Seok, Sa Soo Jin, Hochi Shinichi, Cui Xiang-Shun
Department of Animal Science, Chungbuk National University, Cheongju, Republic of Korea.
Swine Science Division, National Institute of Animal Science, Cheonan-si, Republic of Korea.
Front Cell Dev Biol. 2023 Oct 30;11:1238546. doi: 10.3389/fcell.2023.1238546. eCollection 2023.
Y-box binding protein 1 () plays important roles in RNA stabilization, translation, transcriptional regulation, and mitophagy. However, its effects on porcine preimplantation embryos remain unclear. In this study, we knocked down in the one-cell (1C) stage embryo via small interfering RNA microinjection to determine its function in porcine embryo development. The mRNA level of was found to be highly expressed at the four-cell (4C) stage in porcine embryos compared with one-cell (1C) and two-cell (2C) stages. The number of blastocysts was reduced following YBX1 knockdown. Notably, knockdown decreased the phosphatase and tensin homolog-induced kinase 1 () and parkin RBR E3 ubiquitin protein ligase () mRNA levels. knockdown also decreased PINK1, active mitochondria, and sirtuin 1 levels, indicating reduced mitophagy and mitochondrial biogenesis. Furthermore, knockdown increased the levels of glucose-regulated protein 78 (GRP78) and calnexin, leading to endoplasmic reticulum (ER) stress. Additionally, knockdown increased autophagy and apoptosis. In conclusion, knockdown of decreases mitochondrial function, while increasing ER stress and autophagy during embryonic development.
Y盒结合蛋白1（YBX1）在RNA稳定、翻译、转录调控和线粒体自噬中发挥重要作用。然而，其对猪植入前胚胎的影响仍不清楚。在本研究中，我们通过显微注射小干扰RNA在单细胞（1C）期胚胎中敲低YBX1，以确定其在猪胚胎发育中的功能。与单细胞（1C）和二细胞（2C）期相比，发现YBX1的mRNA水平在猪胚胎的四细胞（4C）期高表达。YBX1敲低后囊胚数量减少。值得注意的是，YBX1敲低降低了磷酸酶和张力蛋白同源物诱导激酶1（PINK1）和帕金RBR E3泛素蛋白连接酶（Parkin）的mRNA水平。YBX1敲低还降低了PINK1、活性线粒体和沉默调节蛋白1的水平，表明线粒体自噬和线粒体生物发生减少。此外，YBX1敲低增加了葡萄糖调节蛋白78（GRP78）和钙连接蛋白的水平，导致内质网（ER）应激。另外，YBX1敲低增加了自噬和凋亡。总之，YBX1敲低在胚胎发育过程中降低线粒体功能，同时增加内质网应激和自噬。
