Shida H
Mol Cell Biol. 1986 Nov;6(11):3734-45. doi: 10.1128/mcb.6.11.3734-3745.1986.
Two classes of revertants were isolated from a vaccinia virus mutant whose hemagglutinins (HAs) accumulate on nuclear envelopes and rough endoplasmic reticulums. The HAs of one of the revertants had the same phenotype as the wild type, i.e., rapid and efficient movement to the cell surface. The HAs of the second class had biphasic transport: rapid export to the cell surface as in the wild type and slow movement to the medial cisternae of the Golgi apparatus. Biochemical and nucleotide sequence analyses showed that the HAs of all the mutants examined that have defects in transport from the rough endoplasmic reticulum to the Golgi apparatus have altered cytoplasmic domains and that the HAs of the second class of revertants lack the whole cytoplasmic domain, while the HAs of the first class of revertants have a wild-type cytoplasmic domain.
从一种血凝素（HA）聚集在核膜和糙面内质网上的痘苗病毒突变体中分离出了两类回复突变体。其中一类回复突变体的HA具有与野生型相同的表型，即快速高效地转运到细胞表面。第二类回复突变体的HA具有双相转运：如野生型一样快速输出到细胞表面，以及缓慢转运到高尔基体的中间潴泡。生化和核苷酸序列分析表明，所有在从糙面内质网到高尔基体的转运过程中存在缺陷的被检测突变体的HA，其胞质结构域都发生了改变，第二类回复突变体的HA缺少整个胞质结构域，而第一类回复突变体的HA具有野生型胞质结构域。
