Westfall T C, Perry H
Neurosci Lett. 1986 Nov 21;71(3):340-4. doi: 10.1016/0304-3940(86)90644-0.
The effect of the nicotinic agonist, dimethylphenylpiperazinium (DMPP), to release endogenous dopamine (DA) from striatal slices obtained from animals chronically treated with the nicotinic agonist for 7 or 14 days was determined. Endogenous DA was measured by high pressure liquid chromatography coupled to electrochemical detection. There was no difference in the DMPP concentration-effect curve for DA release from slices obtained from untreated rats or rats treated with saline. Treatment with DMPP for 7 days did not change the DMPP concentration-effect curve when compared to saline treatment. In contrast, treatment of rats with DMPP for 14 days resulted in a significant attenuation of the DMPP-induced release of DA. These studies support the hypothesis that chronic treatment with nicotinic agonists results in a functional uncoupling of cholinergic nicotinic receptors facilitating DA release in the striatum.
测定了烟碱激动剂二甲基苯基哌嗪鎓（DMPP）对从经烟碱激动剂慢性处理7天或14天的动物获得的纹状体切片中释放内源性多巴胺（DA）的作用。通过高压液相色谱结合电化学检测来测量内源性DA。从未经处理的大鼠或用盐水处理的大鼠获得的切片中，DA释放的DMPP浓度-效应曲线没有差异。与盐水处理相比，用DMPP处理7天并没有改变DMPP浓度-效应曲线。相反，用DMPP处理大鼠14天导致DMPP诱导的DA释放显著减弱。这些研究支持以下假设：烟碱激动剂的慢性处理导致胆碱能烟碱受体功能解偶联，从而促进纹状体中DA的释放。
