十二指肠-空肠旁路术通过调节 ceRNA 网络改善代谢并重塑细胞外基质。

Duodenal-Jejunal bypass improves metabolism and re-models extra cellular matrix through modulating ceRNA network.

机构信息

Department of Clinical Pharmacy, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200020, China; Shanghai Key Laboratory of Pancreatic Disease, Shanghai Jiao Tong University School of Medicine, Shanghai 201620, China.

Department of Pharmacy, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, 2800 Gongwei Road, Pudong, Shanghai 201399, China; Department of Pharmacy, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

出版信息

Genomics. 2023 Nov;115(6):110744. doi: 10.1016/j.ygeno.2023.110744. Epub 2023 Nov 14.

DOI:10.1016/j.ygeno.2023.110744

PMID:37972907

Abstract

BACKGROUND

Bariatric surgery (BS) is an effective approach in treating obesity and ameliorating T2DM with obesity. Our previous studies demonstrated that duodenal-jejunal bypass (DJB) altered long non-coding RNAs (lncRNAs) in the gastrointestinal system, which is associated with modulation of lipid metabolism, and glycemic control through entero-pancreatic axis and gut-brain axis. The adipose non-coding RNA expression profile and the underlying competing endogenous RNA (ceRNA) regulatory network pattern post DJB needs further research and investigation.

RESULTS

In this study, we compared the lncRNAs, circular RNAs (circRNAs) and messenger RNAs (mRNAs) expression in adipose tissues between the sham group and the DJB group. 2219 differentially expressed mRNAs (DEmRNAs), 722 differential expression of lncRNAs (DElncRNAs) and 425 differential expression of circRNAs (DEcircRNAs) were identified. GO terms and KEGG pathways analysis of the DEmRNAs implied that the dysregulated adipose mRNAs were associated with lipid, amino acid metabolism, insulin resistance, and extra cellular matrix (ECM)-related pathways. Moreover, via analyzing ceRNA regulatory networks of DElncRNAs and DEcircRNAs, 31 hub DE mRNAs, especially Mpp7, 9330159F19Rik, Trhde. Trdn, Sorbs2, were found on these pathways.

CONCLUSIONS

The role of DJB in adipose tends to remodel ECM and improve the energy metabolism through the ceRNA regulatory network.

摘要

背景

减重手术（BS）是治疗肥胖症和改善肥胖 2 型糖尿病的有效方法。我们之前的研究表明，十二指肠空肠旁路（DJB）改变了胃肠道中的长链非编码 RNA（lncRNA），这与通过肠胰轴和肠脑轴调节脂质代谢和血糖控制有关。DJB 后脂肪非编码 RNA 表达谱和潜在的竞争性内源性 RNA（ceRNA）调节网络模式需要进一步研究和调查。

结果

在这项研究中，我们比较了假手术组和 DJB 组脂肪组织中的 lncRNA、环状 RNA（circRNA）和信使 RNA（mRNA）表达。鉴定出 2219 个差异表达的 mRNA（DEmRNA）、722 个差异表达的长链非编码 RNA（DElncRNA）和 425 个差异表达的环状 RNA（DEcircRNA）。DEmRNA 的 GO 术语和 KEGG 通路分析表明，失调的脂肪 mRNA 与脂质、氨基酸代谢、胰岛素抵抗和细胞外基质（ECM）相关通路有关。此外，通过分析 DElncRNA 和 DEcircRNA 的 ceRNA 调节网络，发现了 31 个枢纽 DEmRNA，特别是 Mpp7、9330159F19Rik、Trhde、Trdn、Sorbs2，这些都与这些通路有关。