来自挪威污水中的耐替加环素肺炎克雷伯菌菌株携带编码接合质粒的重金属抗性基因。
Tigecycline-resistant Klebsiella pneumoniae strains from sewage in Norway carry heavy-metal resistance genes encoding conjugative plasmids.
作者信息
Radisic Vera, Salvà-Serra Francisco, Moore Edward R B, Marathe Nachiket P
机构信息
Department of Contaminants and Biohazards, Institute of Marine Research (IMR), Bergen, Norway; Department of Biological Sciences, University of Bergen, Bergen, Norway.
Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Department of Clinical Microbiology, Sahlgrenska University Hospital, Region Västra Götaland, Gothenburg, Sweden; Culture Collection University of Gothenburg (CCUG), Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden; Centre for Antibiotic Resistance Research (CARe), University of Gothenburg, Gothenburg, Sweden; Microbiology, Department of Biology, University of the Balearic Islands, Palma de Mallorca, Spain.
出版信息
J Glob Antimicrob Resist. 2024 Mar;36:482-484. doi: 10.1016/j.jgar.2023.10.023. Epub 2023 Nov 14.
OBJECTIVES
Tigecycline is a last-resort antibiotic used for treatment of infections with carbapenem-resistant Klebsiella pneumoniae. The aim of the study was to understand the genetic mechanism of resistance and the genetic context of resistance genes in two tigecycline-resistant K. pneumoniae strains isolated from sewage in Bergen, Norway.
METHODS
Complete genome sequencing of the two strains was accomplished using a combination of short-read Illumina MiSeq-based and long-read Oxford Nanopore MinION-based sequencing. Conjugation experiments were performed using filter mating and a green fluorescent protein (GFP)-tagged Escherichia coli strain.
RESULTS
The complete genome sequences of strain K6-320.1 and strain K7-325 were assembled into two contigs for each strain, one contig representing the complete circular chromosomes of 5 223 440 bp (K6-320.1) and 5 263 092 bp (K7-325), respectively, and the other representing plasmids with sizes of 276 509 bp (pK6-320.1) and 246 731 bp (pK7-325). Strain K6-320.1 belonged to sequence type (ST)869, whereas strain K7-325 belonged to the pathogenic ST307. Both plasmids belonged to the IncFIB(K)/IncFII(K) group and carried several antibiotic resistance genes (ARGs), including tet(A) and bla. Both plasmids (pK6-320.1 and pK7-325) were transferred to a GFP-tagged E. coli strain, leading to the acquisition of resistance against multiple classes of antibiotics. Several heavy-metal resistance genes (HMRGs) encoding resistance against silver (sil), copper (pco), and arsenic (ars) were also present on both plasmids.
CONCLUSIONS
Our study demonstrates the presence of multidrug-resistant K. pneumoniae strains carrying conjugative plasmids encoding both ARGs and HMRGs that have potential for persistence in the environment and human microbiota.
目的
替加环素是用于治疗耐碳青霉烯类肺炎克雷伯菌感染的一种挽救性抗生素。本研究的目的是了解从挪威卑尔根污水中分离出的两株耐替加环素肺炎克雷伯菌菌株的耐药遗传机制以及耐药基因的遗传背景。
方法
使用基于短读长的Illumina MiSeq测序和基于长读长的牛津纳米孔MinION测序相结合的方法完成了这两株菌株的全基因组测序。使用滤膜交配法和绿色荧光蛋白（GFP）标记的大肠杆菌菌株进行了接合实验。
结果
菌株K6-320.1和菌株K7-325的全基因组序列分别组装成两个重叠群，一个重叠群分别代表大小为5223440 bp（K6-320.1）和5263092 bp（K7-325）的完整环状染色体，另一个代表大小分别为276509 bp（pK6-320.1）和246731 bp（pK7-325）的质粒。菌株K6-320.1属于序列型（ST）869，而菌株K7-325属于致病性ST307。两种质粒均属于IncFIB(K)/IncFII(K)组，并携带多个抗生素抗性基因（ARGs），包括tet(A)和bla。两种质粒（pK6-320.1和pK7-325）均转移至GFP标记的大肠杆菌菌株，导致该菌株获得对多类抗生素的抗性。两种质粒上还存在几个编码对银（sil）、铜（pco）和砷（ars）抗性的重金属抗性基因（HMRGs）。
结论
我们的研究证明了存在携带编码ARGs和HMRGs的接合质粒的多重耐药肺炎克雷伯菌菌株，这些菌株在环境和人类微生物群中具有持续存在的可能性。
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