利用快速冷冻深蚀刻电子显微镜观察密旋链霉菌孢子囊和游动孢子的结构。
Architecture of Actinoplanes missouriensis sporangia and zoospores visualized using quick-freeze deep-etch electron microscopy.
机构信息
Department of Biotechnology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Tokyo, Japan.
Department of Biology, Graduate School of Science, Osaka Metropolitan University, Osaka, Japan.
出版信息
Biosci Biotechnol Biochem. 2024 Jan 24;88(2):225-229. doi: 10.1093/bbb/zbad159.
The architecture of sporangia and zoospores of Actinoplanes missouriensis was analyzed at a high resolution using quick-freeze deep-etch replica electron microscopy. This analysis revealed that (i) sporangia were surrounded by at least 2 membranous layers with smooth surfaces, (ii) zoospores were enclosed by a fibrillar layer, and (iii) flagella were generated in a restricted area on the zoospore surface.
采用快速冷冻深蚀刻复型电子显微镜技术对 Missouriensis 游动放线菌的游动孢子囊和游动孢子的结构进行了高分辨率分析。该分析表明：（i）游动孢子囊至少被 2 层具有光滑表面的膜层所包围；（ii）游动孢子被一层纤维层所包裹；（iii）鞭毛在游动孢子表面的一个受限区域产生。
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