利用从传统乳制品中分离出的潜在益生菌菌株植物乳杆菌 MK06 治疗牙龈炎的切实前景：一项三盲随机临床试验。

A tangible prospect for the treatment of gingivitis using a potentially probiotic strain Lactobacillus plantarum MK06 isolated from traditional dairy products: a triple blind randomized clinical trial.

机构信息

National Institute of Genetic Engineering and Biotechnology (NIGEB), P. O. Box 14155-6343, Tehran, Iran.

Department of Periodontology, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.

出版信息

BMC Oral Health. 2023 Nov 16;23(1):870. doi: 10.1186/s12903-023-03494-x.

DOI:10.1186/s12903-023-03494-x

PMID:37974136

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10655419/

Abstract

BACKGROUND

Frequent bacterial plaque buildup at the gingival margin and crevice can provoke an inflammatory reaction in gingival tissues which manifests as gingivitis. Probiotics could serve as a beneficial complementary therapy for treating gingival inflammation. The main aim of this research was to investigate the effect of the Lactobacillus plantarum MK06 probiotic strain on the treatment of gingivitis.

METHODS

Patients with gingivitis, who were referred to a private clinic and were systematically healthy, were included in this randomized, triple-blind, placebo-controlled trial. They were instructed to use either placebo or Lactobacillus plantarum suspensions for one minute two times a day after tooth-brushing for four weeks. Then, the clinical parameters of gingivitis, including plaque index (PI), gingival index (GI), bleeding on probing (BOP), and oral hygiene index (OHI-s), were measured in the first, second, and fourth weeks. A total of forty-two patients were randomly assigned to the experimental (n = 21) and control (n = 21) groups. The mean age of the experimental and control groups was 29.10 and 28.48, respectively.

RESULTS

The mean scores of BOP, GI, PI, and OHI-s reduced over time in both the control and test groups. However, according to the Mann-Whitney test, the difference between the two groups was not significant at the same time intervals (P ≥ 0.05) and only GI showed a significant difference in the fourth week (GI-3, P = 0.006). Nevertheless, the experimental group experienced a higher overall reduction rate than the control group. The BOP, GI, PI, and OHI-s scores decreased by 0.081, 0.204, 0.186, and 0.172 times in the second week, respectively, resulting from the interaction of time and the intervention, which considerably diminished these indices.

CONCLUSION

This study shows the potential of the probiotic Lactobacillus plantarum MK06 suspension as a promoting therapeutic adjuvant in the treatment of gingivitis.

摘要

背景

牙龈边缘和缝隙处频繁的细菌菌斑堆积会引发牙龈组织的炎症反应，表现为牙龈炎。益生菌可以作为治疗牙龈炎症的有益补充疗法。本研究的主要目的是研究植物乳杆菌 MK06 益生菌株对治疗牙龈炎的效果。

方法

本随机、三盲、安慰剂对照试验纳入了因牙龈炎到私人诊所就诊且系统健康的患者。他们被指导在刷牙后使用安慰剂或植物乳杆菌混悬液，每天两次，每次一分钟，持续四周。然后，在第一、第二和第四周测量牙龈炎的临床参数，包括菌斑指数（PI）、牙龈指数（GI）、探诊出血（BOP）和口腔卫生指数（OHI-s）。共有 42 名患者被随机分配到实验组（n=21）和对照组（n=21）。实验组和对照组的平均年龄分别为 29.10 岁和 28.48 岁。

结果

在对照组和试验组中，BOP、GI、PI 和 OHI-s 的平均评分随时间推移而降低。然而，根据曼-惠特尼检验，在同一时间间隔内两组之间的差异不显著（P≥0.05），仅在第四周时 GI 有显著差异（GI-3，P=0.006）。尽管如此，实验组的整体降低率高于对照组。在第二周时，BOP、GI、PI 和 OHI-s 的评分分别降低了 0.081、0.204、0.186 和 0.172 倍，这是由于时间和干预的相互作用，大大降低了这些指数。