School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Feb 15;307:123638. doi: 10.1016/j.saa.2023.123638. Epub 2023 Nov 10.
The detection of residual tetracycline antibiotics (TCs) in the aquatic environment is important because of the irreversible damage of TCs to the ecosystem and human health. Considering the photostability, sensitivity, and accuracy of the detection systems, mesoporous SBA-15 silica material was an ideal support matrix for improving photostability because it can protect the optical properties of fluorescent signals. To achieve ratiometric detection (with sensitivity and accuracy), pyrene and Eu as two fluorescent signals were implemented into SBA-15, and an organic-inorganic hybrid fluorescent nanoprobe Py/EDTA-SBA-15@Eu was constructed. In the presence of TCs, the red emission of Eu in the nanoprobe was sensitized, and the fluorescence resonance energy transfer occurred from pyrene to Eu, resulting in the increased emission of Eu at 617 nm and the decreased emission of pyrene at 384 nm. By monitoring the fluorescent intensity changes at 617 and 384 nm (F/F), the nanoprobe achieved the ratiometric detection of oxytetracycline (OTC) and tetracycline (TC) in the range of 0.20-80 and 0.40-80 μM, respectively, with detection limits of 0.18 nM for OTC and 5.83 nM for TC. In addition, the practical application of the nanoprobe was validated in tap water and honey samples.
检测水体中残留的四环素类抗生素（TCs）很重要，因为 TCs 会对生态系统和人类健康造成不可逆转的损害。考虑到检测系统的光稳定性、灵敏度和准确性，介孔 SBA-15 硅材料是一种理想的支撑基质，可以提高光稳定性，因为它可以保护荧光信号的光学性质。为了实现比率检测（具有灵敏度和准确性），将芘和 Eu 作为两种荧光信号引入 SBA-15 中，并构建了有机-无机杂化荧光纳米探针 Py/EDTA-SBA-15@Eu。在 TCs 的存在下，纳米探针中 Eu 的红色发射被敏化，并且发生了从芘到 Eu 的荧光共振能量转移，导致 Eu 在 617nm 处的发射增加，而芘在 384nm 处的发射减少。通过监测 617nm 和 384nm 处的荧光强度变化（F/F），纳米探针实现了在 0.20-80 和 0.40-80μM 范围内对土霉素（OTC）和四环素（TC）的比率检测，其对 OTC 的检测限为 0.18nM，对 TC 的检测限为 5.83nM。此外，还验证了纳米探针在自来水和蜂蜜样品中的实际应用。
