Department of Food Science and Nutrition, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region; Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region; Research Institute for Future Food, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region.
Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hong Kong Special Administrative Region; Centre for Eye and Vision Research (CEVR), 17W Hong Kong Science Park, Hong Kong Special Administrative Region.
Food Chem. 2024 Mar 30;437(Pt 2):137953. doi: 10.1016/j.foodchem.2023.137953. Epub 2023 Nov 7.
Ellagic acid (EA) is an abundant dietary polyphenol with multifarious health benefits but low bioavailability. To increase its bio-efficacy, converting EA to urolithins by supplementing urolithin-producing bacteria, e.g., Gordonibacter urolithinfaciens (G.uro), could be a solution. This work first tested three methods for oral delivery of live G.uro. Intestinal colonization of G.uro and its impact on local gut microbiota, EA-to-urolithin conversion and bioavailability were then investigated in C57BL/6J mice administered to EA only or a synbiotic (G. uro + EA). In vitro results suggested that G.uro largely survived simulated gastrointestinal digestion and could be administered without protection. In vivo results showed that G.uro had little impact on local gut microbiota but could not colonize the gut. Moreover, synbiotic remarkably promoted Akkermansia, Lactobacillus and Bifidobacterium growth, and significantly enhanced the bioavailability of urolithins compared with the EA-only group. This study demonstrated the potential of oral supplementation of G.uro for enhancing EA-to-urolithin bioconversion and bioavailability.
鞣花酸（EA）是一种丰富的膳食多酚，具有多种健康益处，但生物利用度低。为了提高其生物功效，可以通过补充产生尿石素的细菌（例如戈登氏菌）将 EA 转化为尿石素。本工作首先测试了三种口服递送活戈登氏菌的方法。然后在给予 EA 或共生菌（戈登氏菌+EA）的 C57BL/6J 小鼠中研究了 G.uro 的肠道定植及其对局部肠道微生物群、EA 到尿石素的转化和生物利用度的影响。体外结果表明，G.uro 在模拟胃肠道消化过程中大量存活，无需保护即可给药。体内结果表明，G.uro 对局部肠道微生物群几乎没有影响，但不能定植肠道。此外，与 EA 单药组相比，共生菌显著促进了阿克曼氏菌、乳杆菌和双歧杆菌的生长，并显著提高了尿石素的生物利用度。这项研究证明了口服补充戈登氏菌增强 EA 到尿石素生物转化和生物利用度的潜力。
