State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing 100084, China.
Sci Total Environ. 2024 Feb 20;912:168620. doi: 10.1016/j.scitotenv.2023.168620. Epub 2023 Nov 17.
With the increasing complexity of influent composition in wastewater treatment plants, the potential stimulating effects of refractory organic matter in wastewater on growth characteristics and genera conversion of nitrifying bacteria (ammonium-oxidizing bacteria [AOB] and nitrite-oxidizing bacteria [NOB]) need to be further investigated. In this study, domestic wastewater was co-treated with landfill leachate in the lab-scale reactor, and the competition and co-existence of NOB genera Nitrotoga and Nitrospira were observed. The results demonstrated that the addition of landfill leachate could induce the growth of Nitrotoga, whereas Nitrotoga populations remain less competitive in domestic wastewater operation. In addition, the refractory organic matter in the landfill leachate also would have a potential stimulating effect on the maximum specific growth rate of AOB genus Nitrosomonas (μ). The μ of Nitrosomonas in the control group was estimated to be 0.49 d by fitting the ASM model, and the μ reached 0.66-0.71 d after injection of refractory organic matter in the landfill leachate, while the maximum specific growth rate of NOB (μ) was always in the range of 1.05-1.13 d. These findings have positive significance for the understanding of potential stimulation on nitrification processes and the stable operation of innovative wastewater treatment process.
随着污水处理厂进水中复杂有机物的不断增加，需要进一步研究废水中难降解有机物对硝化菌（氨氧化菌[AOB]和亚硝酸盐氧化菌[NOB]）生长特性和菌群转化的潜在刺激作用。本研究在实验室规模的反应器中用渗滤液共处理生活污水，观察了 NOB 属硝化螺菌和亚硝化单胞菌之间的竞争和共存。结果表明，添加渗滤液会诱导硝化螺菌的生长，而在生活污水运行中硝化螺菌的种群竞争力仍然较低。此外，渗滤液中的难降解有机物也可能对 AOB 属亚硝化单胞菌（μ）的最大比生长速率产生潜在的刺激作用。通过拟合 ASM 模型，对照组中亚硝化单胞菌的μ估计为 0.49 d，注入渗滤液中的难降解有机物后，μ达到 0.66-0.71 d，而 NOB（μ）的最大比生长速率始终在 1.05-1.13 d 的范围内。这些发现对理解硝化过程的潜在刺激作用以及创新污水处理工艺的稳定运行具有积极意义。
