Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Tai'an City, Shandong Province 271018, China.
Sino-German Cooperative Research Centre for Zoonosis of Animal Origin of Shandong Province, Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, 61 Daizong Street, Tai'an City, Shandong Province 271018, China; School of Clinical and Basic Medical Sciences, Shandong First Medical University & Shandong Academy of Medical Sciences, Ji'nan 250024, China.
Vet Immunol Immunopathol. 2023 Nov;265:110666. doi: 10.1016/j.vetimm.2023.110666. Epub 2023 Nov 4.
China is the country with the largest amount of duck breeding as well as duck meat and egg production. In recent years, the emergence and spread of duck Tembusu virus (DTMUV) has become one of the important factors in reducing the amount of duck slaughter, which seriously endangers the duck breeding industry in our country. In-depth research on the mechanism of duck innate immunity facilitates the exploration of new models for the treatment of DTMUV infection. IRF1 can induce the expression of many antiviral immune factors in the animal organism and play an important role in the innate immune response. In this study, we used interfering RNA to knock down the IRF1 gene in DEF cells and then the cells were infected with DTMUV. We found that knockdown of IRF1 promoted DTMUV replication at an early stage and caused downregulation of the expression of several major pattern recognition receptors (PRRs), interleukins (IL), interferons (IFN), antiviral proteins, and MHC molecules by assay, showing that the duIRF1-mediated signaling pathway plays an extremely important role in DTMUV-induced host innate immunity. In addition, we constructed the recombinant expression plasmid pET32a(+)-duIRF1-His, and finally prepared the polyclonal antibody of duIRF1 with good specificity, hoping to provide a detection means for research on the mechanism of IRF1 in innate immunity in our laboratory and in this field.
中国是养鸭数量最多、鸭肉和禽蛋产量最大的国家。近年来，鸭坦布苏病毒（DTMUV）的出现和传播已成为减少鸭屠宰量的重要因素之一，这严重威胁了我国的养鸭业。深入研究鸭固有免疫机制有助于探索治疗 DTMUV 感染的新模型。IRF1 可以诱导动物机体中许多抗病毒免疫因子的表达，在固有免疫反应中发挥重要作用。在本研究中，我们使用干扰 RNA 敲低 DEF 细胞中的 IRF1 基因，然后用 DTMUV 感染细胞。我们发现，IRF1 的敲低促进了 DTMUV 在早期的复制，并导致几种主要的模式识别受体（PRRs）、白细胞介素（IL）、干扰素（IFN）、抗病毒蛋白和 MHC 分子的表达下调，表明 duIRF1 介导的信号通路在 DTMUV 诱导的宿主固有免疫中起着极其重要的作用。此外，我们构建了重组表达质粒 pET32a(+)-duIRF1-His，并最终制备了具有良好特异性的 duIRF1 多克隆抗体，希望为我们实验室和该领域研究 IRF1 在固有免疫中的机制提供一种检测手段。
