Zhang Qiumei, Li Yujiao, Fan Bei, Wang Fengzhong, Li Zhi, Pires Dias Alberto Carlos, Liu Xinmin, Wang Qiong
Sino-Portugal TCM International Cooperation Center, The Affiliated Traditional Chinese Medicine Hospital, Southwest Medical University, Luzhou, 646000, China; Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
J Ethnopharmacol. 2024 Apr 24;324:117416. doi: 10.1016/j.jep.2023.117416. Epub 2023 Nov 21.
Dendrobium nobile Lindl (DNL), a valued time-honored herb, possesses immune-boosting and age-delaying properties, has been widely used to treat hyperglycemia and neurological diseases, and is probably a potential drug for improving learning and memory. Scopolamine (Scop), an antagonist for muscarinic receptors, potentially impairing intelligence and memory.
This investigation aimed to assess the efficacy of DNL in alleviating scopolamine-induced cognitive deficits in mice and its mechanisms.
We utilized the open-field test, novel object recognition test (NOR), and Morris water maze test (MWM) to assess the potential of DNL in ameliorating learning and memory dysfunction caused by scopolamine in mice. Enzyme-linked immunosorbent assay (ELISA) was employed to measure Choline acetyltransferase (ChAT) content and Acetylcholinesterase (AChE) activities in the brain, and oxidative stress-related factors in the serum, including Malondialdehyde (MDA), Superoxide dismutase (SOD), and glutathione (GSH) content.
Scopolamine injection significantly reduced the discrimination index of mice in the NOR test and impaired their performance in the MWM test, as demonstrated by longer escape latency, fewer target crossings, and less time spent in the target quadrant in the MWM. After 25 days of administration, DNL increased the discrimination index of the scopolamine-treated mice in the NOR test. DNL reduced the escape latency in the MWM test in the model mice. DNL increased the target crossing number and the percentage of time spent in the target quadrant in the MWM test. ELISA experiments indicated that DNL decreased the AChE activities, increased the ChAT activities, and modulated oxidative stress makers (GSH, SOD, and MDA) in scopolamine-induced mice.
DNL may improve the learning and memory in mice treated with scopolamine, possibly by modulating oxidative stress and impaired cholinergic function.
铁皮石斛是一种珍贵的名贵草药，具有增强免疫力和延缓衰老的特性，已被广泛用于治疗高血糖和神经系统疾病，可能是一种改善学习和记忆的潜在药物。东莨菪碱是一种毒蕈碱受体拮抗剂，可能损害智力和记忆。
本研究旨在评估铁皮石斛减轻东莨菪碱诱导的小鼠认知缺陷的功效及其机制。
我们利用旷场试验、新物体识别试验（NOR）和莫里斯水迷宫试验（MWM）来评估铁皮石斛改善东莨菪碱引起的小鼠学习和记忆功能障碍的潜力。采用酶联免疫吸附测定（ELISA）法检测大脑中胆碱乙酰转移酶（ChAT）含量和乙酰胆碱酯酶（AChE）活性，以及血清中氧化应激相关因子，包括丙二醛（MDA）、超氧化物歧化酶（SOD）和谷胱甘肽（GSH）含量。
注射东莨菪碱显著降低了小鼠在NOR试验中的辨别指数，并损害了它们在MWM试验中的表现，MWM试验中表现为逃避潜伏期延长、目标象限穿越次数减少和在目标象限停留时间缩短。给药25天后，铁皮石斛增加了东莨菪碱处理小鼠在NOR试验中的辨别指数。铁皮石斛缩短了模型小鼠在MWM试验中的逃避潜伏期。铁皮石斛增加了MWM试验中目标象限穿越次数和在目标象限停留时间的百分比。ELISA实验表明，铁皮石斛降低了东莨菪碱诱导小鼠的AChE活性，增加了ChAT活性，并调节了氧化应激标志物（GSH、SOD和MDA）。
铁皮石斛可能改善东莨菪碱处理小鼠的学习和记忆，可能是通过调节氧化应激和受损的胆碱能功能来实现的。
