快速检测胸腔积液中的化脓性链球菌和肺炎链球菌，以诊断脓性胸腔积液。
Rapid detection of S. pyogenes and S. pneumoniae in pleural fluid for diagnosis of parapneumonic empyema.
机构信息
Department of Internal Medicine and Infectious Diseases, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
Department of Medical Microbiology, Ghent University Hospital, Corneel Heymanslaan 10, 9000, Ghent, Belgium.
出版信息
Eur J Clin Microbiol Infect Dis. 2024 Jan;43(1):195-201. doi: 10.1007/s10096-023-04710-w. Epub 2023 Nov 20.
The aim of this study was to assess the reliability of rapid antigen detection tests (RADT) for Streptococcus pyogenes (GAS) and Streptococcus pneumoniae on pleural fluid samples for diagnosis of parapneumonic effusion/empyema (PPE) and their potential for improving pathogen identification rates. Sixty-three pleural samples were included from 54 patients on which GAS and S. pneumoniae RADT (BinaxNOW), culture, 16S rRNA PCR, and S. pneumoniae-specific PCR were performed. GAS RADT showed a sensitivity of 95.2% and a specificity of 100%. Pneumococcal RADT showed a sensitivity of 100% and specificity of 88.6%. Both RADT increased the pathogen identification rate in PPE compared to culture.
本研究旨在评估快速抗原检测试验（RADT）在胸腔积液样本中对化脓性链球菌（GAS）和肺炎链球菌检测的可靠性，以诊断类肺炎性胸腔积液/脓胸（PPE），并评估其提高病原体鉴定率的潜力。共纳入 54 例患者的 63 份胸腔样本，对这些样本进行 GAS 和肺炎链球菌 RADT（BinaxNOW）、培养、16S rRNA PCR 和肺炎链球菌特异性 PCR 检测。GAS RADT 的敏感性为 95.2%，特异性为 100%。肺炎球菌 RADT 的敏感性为 100%，特异性为 88.6%。与培养相比，两种 RADT 均提高了 PPE 中的病原体鉴定率。
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