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使用泄殖腔镜检查法对澳大利亚石龙子( , , )进行性别鉴定。

Sex identification in Australian skinks (, , ) using cloacoscopy.

作者信息

Knotek Zdenek, Oliveri Matteo, Cermakova Eva, Sramek Petr

机构信息

Avian and Exotic Animal Clinic, Faculty of Veterinary Medicine, University of Veterinary Sciences Brno, Brno, Czech Republic.

Faculty of Veterinary Medicine, Teaching Veterinary Hospital, University of Teramo, Teramo, Italy.

出版信息

Vet Med (Praha). 2023 Jun 20;68(6):253-257. doi: 10.17221/3/2023-VETMED. eCollection 2023 Jun.

DOI:10.17221/3/2023-VETMED
PMID:37982004
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10581510/
Abstract

Thirteen adult healthy captive skinks, six Gidgee spiny-tailed skinks (), three Hosmer's spiny-tailed skinks () and four tree crevice-skinks (), were submitted to the study. The weight of the animals ranged from 28 g to 146 g. All skinks were explored during their putative mating season, which is December. Lizards were restrained manually and positioned in ventral recumbence. Cloacoscopy was performed with the rigid endoscope, protecting channel, endocamera and recording system Telepack-Pal (Karl Storz Endoskope, Tuttlingen, Germany). While the endoscope was gently introduced into the cloaca and advanced into the coprodeum sterile saline was flushed into the cloaca through the protecting channel. The endoscope was then slowly withdrawn to allow visualization of the main structures of the urodeum and proctodeum. Male skinks are characterized by the presence of urethral and only one horizontal septum which divides the chamber of the urodeum into two subchambers. Female skinks are characterised by the presence of two septa. The central-dorsal fold that divides the urodeum into two pouches in female skinks is absent in males. Cloacoscopy proved an effective method of sex identification and can be considered a valuable method for breeding and conservation in monomorphic skink lizards.

摘要

13只成年健康圈养石龙子,6只吉奇棘尾石龙子()、3只霍斯默棘尾石龙子()和4只树缝石龙子()被纳入该研究。动物体重在28克至146克之间。所有石龙子均在其假定的交配季节(即12月)进行检查。蜥蜴被手动固定,呈腹卧位。使用刚性内窥镜、保护通道、内窥摄像头和Telepack-Pal记录系统(德国图特林根卡尔·史托斯内窥镜公司)进行泄殖腔镜检查。当内窥镜轻轻插入泄殖腔并推进到粪道时,无菌生理盐水通过保护通道冲洗入泄殖腔。然后缓慢撤回内窥镜,以便观察尿生殖道和肛道的主要结构。雄性石龙子的特征是存在尿道 且只有一个水平隔膜,该隔膜将尿生殖道腔分为两个子腔。雌性石龙子的特征是存在两个隔膜。雌性石龙子中将尿生殖道分为两个囊袋的中央背侧褶皱在雄性中不存在。泄殖腔镜检查被证明是一种有效的性别鉴定方法,可被视为单态石龙子蜥蜴繁殖和保护的一种有价值的方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/db63740c8491/VETMED-68-123003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/b2dba966b737/VETMED-68-123003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/479981eff553/VETMED-68-123003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/8058f04bd5a6/VETMED-68-123003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/db63740c8491/VETMED-68-123003-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/b2dba966b737/VETMED-68-123003-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/479981eff553/VETMED-68-123003-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/8058f04bd5a6/VETMED-68-123003-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/458f/10581510/db63740c8491/VETMED-68-123003-g004.jpg

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本文引用的文献

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Sex identification in the eastern blue-tongued lizard (Tiliqua scincoides White, ex Shaw, 1790) using morphometrics.利用形态测量法对东部蓝舌石龙子(Tiliqua scincoides White,源于Shaw,1790年)进行性别鉴定。
Aust Vet J. 2016 Jul;94(7):256-9. doi: 10.1111/avj.12429.
2
Diagnostic sensitivity of ultrasound, radiography and computed tomography for gender determination in four species of lizards.超声、X射线摄影及计算机断层扫描对四种蜥蜴性别判定的诊断敏感性
Vet Radiol Ultrasound. 2015 Jan-Feb;56(1):40-5. doi: 10.1111/vru.12186. Epub 2014 Jul 26.