一项关于大肠杆菌DNA聚合酶I制剂催化的未引发聚（dA-dT）合成的研究。
A study on the unprimed poly (dA-dT) synthesis catalyzed by preparations of E. coli DNA polymerase I.
作者信息
Nazarenko I A, Bobko L E, Romashchenko A G, Khripin Y L, Salganik R I
出版信息
Nucleic Acids Res. 1979 Jun 11;6(7):2545-60. doi: 10.1093/nar/6.7.2545.
Evidence was obtained indicating that the initiation of poly (dA-dT) de novo synthesis is provided by deoxynucleoside diphosphate: oligonucleotide deoxynucleotidyl transferase (dNDP-transferase present in preparations of E. coli DNA polymerase I and capable of catalyzing the unprimed polymerization of dNDP. dNDP-transferase synthesyzes short oligonucleotides which form template-primer complexes repeatedly replicated by DNA polymerase I. This conclusion was based on the following observations: the abolition of the lag period of poly (dA-dT) synthesis by preincubation of DNA-polymerase I preparations with dADP and dTDP; the presence of oligo (dA-dT) among the preincubation products; the suppressive effect of dithiothreitol and N-ethylmaleimide (inhibitors of dNDP-transferase) on the de novo, but not on the primed synthesis of poly (dA-dT), catalyzed by preparations of DNA-polymerase I.
已获得的证据表明，聚（dA-dT）的从头合成起始由脱氧核苷二磷酸提供：寡核苷酸脱氧核苷酸转移酶（dNDP-转移酶，存在于大肠杆菌DNA聚合酶I制剂中，能够催化dNDP的无引物聚合。dNDP-转移酶合成短寡核苷酸，这些寡核苷酸形成由DNA聚合酶I反复复制的模板-引物复合物。这一结论基于以下观察结果：通过将DNA聚合酶I制剂与dADP和dTDP预孵育消除聚（dA-dT）合成的延迟期；预孵育产物中存在寡聚（dA-dT）；二硫苏糖醇和N-乙基马来酰亚胺（dNDP-转移酶抑制剂）对DNA聚合酶I制剂催化的聚（dA-dT）的从头合成有抑制作用，但对引物合成无抑制作用。
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