The C. S. Mott Center for Human Growth and Development, Wayne State University School of Medicine, Detroit, Michigan, USA.
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, Michigan, USA.
Biomarkers. 2023 Dec;28(7):663-671. doi: 10.1080/1354750X.2023.2284106. Epub 2023 Dec 11.
Ovarian cancer cells are known to express myeloperoxidase (MPO), an oxidant-producing enzyme with a 150 kDa homodimer, consisting of two identical monomers connected by a disulfide bond. Here, we aim to validate monomeric MPO (mMPO) as a biomarker for the early detection of ovarian cancer. Human ovarian cancer cells, sera from patients at various stages, sera from non-cancer inflammatory gynecological diseases, and healthy volunteers were used. Monomeric and dimeric MPO were measured by ELISA. Receiver operating curves were used to compare the predictive powers of serum dimeric and monomeric MPO to discriminate between samples. The expression of MPO was unique to ovarian cancer cells. Specifically, mMPO was found to be the only form of MPO in all ovarian cancer cell lines. Intriguingly, mMPO was detected in the sera from all patients with ovarian cancer at various stages, but not from healthy individuals. Serum mMPO discriminated between early-stage ovarian cancer, healthy controls, and benign inflammatory gynecologic disorders. In addition, mMPO discriminated between the early and late stages of the disease. This work highlights mMPO as a potential biomarker for early detection of ovarian cancer, which is critically needed.
已知卵巢癌细胞表达髓过氧化物酶（MPO），这是一种产生氧化剂的酶，具有 150kDa 的同型二聚体，由两个通过二硫键连接的相同单体组成。在这里，我们旨在验证单体 MPO（mMPO）作为卵巢癌早期检测的生物标志物。使用人卵巢癌细胞、处于不同阶段的患者血清、非癌症炎症性妇科疾病患者血清和健康志愿者的血清。通过 ELISA 测量单体和二聚体 MPO。使用受试者工作特征曲线比较血清中二聚体和单体 MPO 的预测能力，以区分样本。MPO 的表达是卵巢癌细胞所特有的。具体而言，mMPO 被发现是所有卵巢癌细胞系中唯一形式的 MPO。有趣的是，mMPO 存在于所有处于不同阶段的卵巢癌患者的血清中，但不存在于健康个体中。血清 mMPO 可区分早期卵巢癌、健康对照和良性炎症性妇科疾病。此外，mMPO 可区分疾病的早期和晚期。这项工作强调了 mMPO 作为卵巢癌早期检测的潜在生物标志物的重要性。
