Departments of Stomatology and Central Lab, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, China.
Department of Stomatology, The Dushu Lake Hospital Affiliated to Soochow University, Suzhou, Jiangsu, China.
Oral Dis. 2024 Jul;30(5):3386-3399. doi: 10.1111/odi.14807. Epub 2023 Nov 20.
In this study, we isolated human periodontal ligament cells (hPDLCs) to find the optimal time of LIPUS stimulation and to explore how LIPUS affects inflammatory and osteogenic responses in hPDLCs in an inflammatory environment. The target molecules of LIPUS were identified by high-throughput sequencing. RT-qPCR and WB were used to detect how LIPUS affected the expression of related genes in TNFα-induced inflammation. The expression of ROS and inflammatory factors was detected by flow cytometry. Immunohistochemistry was used to further verify gene expression in rats. hPDLCs were isolated successfully. The optimal LIPUS stimulation condition was 45 mW/cm for 30 min and continued for 3 days, and this intensity significantly promoted the osteogenesis and mineralization of hPDLCs. LIPUS significantly inhibited the upregulation of IL-6 and ROS, increased the percentage of cells in the G2 phase, inhibited cell apoptosis, and inhibited the upregulation of TLR5 expression in an inflammatory environment. LIPUS can effectively restrain the inflammation and oxidative stress response of hPDLCs and promote osteogenesis in an inflammatory environment. LIPUS inhibited the periodontal inflammatory response through TLR5 in hPDLCs and dental pulp.
在这项研究中，我们分离了人牙周膜细胞（hPDLCs），以找到 LIPUS 刺激的最佳时间，并探讨 LIPUS 如何在炎症环境中影响 hPDLCs 的炎症和成骨反应。通过高通量测序鉴定 LIPUS 的靶分子。使用 RT-qPCR 和 WB 检测 LIPUS 如何影响 TNFα 诱导的炎症中相关基因的表达。通过流式细胞术检测 ROS 和炎症因子的表达。免疫组织化学进一步验证大鼠中的基因表达。成功分离出 hPDLCs。最佳 LIPUS 刺激条件为 45mW/cm2，持续 30 分钟，共 3 天，这种强度显著促进了 hPDLCs 的成骨和矿化。LIPUS 显著抑制了 IL-6 和 ROS 的上调，增加了 G2 期细胞的百分比，抑制了细胞凋亡，并抑制了 TLR5 表达在炎症环境中的上调。LIPUS 可有效抑制 hPDLCs 炎症和氧化应激反应，并促进炎症环境中的成骨作用。LIPUS 通过 hPDLCs 和牙髓中的 TLR5 抑制牙周炎症反应。
