脑桥臂旁核-基底前脑皮层和海马唤醒调节回路。
Pontine parabrachial nucleus-basal forebrain circuitry regulating cortical and hippocampal arousal.
机构信息
Department of Neurology and Neuroscience Center, The First Hospital of Jilin University, Changchun, Jilin, China; Department of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Department of Neurology, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA; Ningxia Key Laboratory of Craniocerebral Diseases, Department of Anatomy, Histology and Embryology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia, China.
出版信息
Sleep Med. 2024 Jan;113:49-55. doi: 10.1016/j.sleep.2023.10.032. Epub 2023 Nov 10.
INTRODUCTION
The basal forebrain (BF) and the medial septum (MS) respectively drive neuronal activity of cerebral cortex and hippocampus (HPC) in sleep-wake cycle. Our previous studies of lesions and neuronal circuit tracing have shown that the pontine parabrachial nucleus (PB) projections to the BF and MS may be a key circuit for cortical and HPC arousal.
AIMS
This study aims to demonstrate that PB projections to the BF and MS activate the cerebral cortex and HPC.
RESULTS
By using chemogenetic stimulation of the BF, the PB-BF and the PB-MS pathway combined with electroencephalogram (EEG) Fast Fourier Transformation (FFT) analysis in rats, we demonstrated that chemogenetic stimulation of the BF or PB neurons projecting to the BF activated the cerebral cortex while chemogenetic stimulation of the MS or PB neurons projecting to the MS activated HPC activity, in sleep and wake state. These stimulations did not significantly alter sleep-wake amounts.
CONCLUSIONS
Our results support that PB projections to the BF and MS specifically regulating cortical and HPC activity.
简介
基底前脑 (BF) 和中隔 (MS) 分别驱动睡眠-觉醒周期中大脑皮层和海马体 (HPC) 的神经元活动。我们之前关于损伤和神经元回路追踪的研究表明，桥脑臂旁核 (PB) 投射到 BF 和 MS 的神经元可能是皮质和 HPC 觉醒的关键回路。
目的
本研究旨在证明 PB 投射到 BF 和 MS 的神经元可激活大脑皮层和 HPC。
结果
通过使用化学遗传刺激 BF，结合大鼠脑电图 (EEG) 快速傅里叶变换 (FFT) 分析，我们证明了化学遗传刺激 BF 或投射到 BF 的 PB 神经元激活了大脑皮层，而化学遗传刺激 MS 或投射到 MS 的 PB 神经元则激活了 HPC 活动，无论在睡眠还是觉醒状态下。这些刺激并没有显著改变睡眠-觉醒的量。
结论
我们的结果支持 PB 投射到 BF 和 MS 的神经元特异性调节皮质和 HPC 的活动。
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