Hainan Yazhou Bay Seed Laboratory, Sanya Nanfan Research Institute of Hainan University, Sanya, China.
College of Tropical Crops, Hainan University, Haikou, China.
Plant Cell Environ. 2024 Apr;47(4):1023-1040. doi: 10.1111/pce.14770. Epub 2023 Nov 20.
Drought stress poses a persistent threat to field crops and significantly limits global agricultural productivity. Plants employ ubiquitin-dependent degradation as a crucial post-translational regulatory mechanism to swiftly adapt to changing environmental conditions. JUL1 is a RING-type E3 ligase related to drought stress in Arabidopsis. In this study, we explored the function of BnaJUL1 (a homologous gene of JUL1 in Brassica napus) and discovered a novel gene BnaTBCC1 participating in drought tolerance. First, we utilised BnaJUL1-cri materials through the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated protein 9 system. Second, we confirmed that BnaJUL1 regulated drought tolerance through the drought tolerance assay and transcriptome analysis. Then, we identified a series of proteins interacting with BnaJUL1 through yeast library screening, including BnaTBCC1 (a tubulin binding cofactor C domain-containing protein); whose homologous gene TBCC1 knockdown mutants (tbcc1-1) exhibited ABA-sensitive germination in Arabidopsis, we then confirmed the involvement of BnaTBCC1 in drought tolerance in both Arabidopsis and Brassica. Finally, we established that BnaJUL1 could ubiquitinate and degrade BnaTBCC1 to regulate drought tolerance. Consequently, our study unveils BnaJUL1 as a novel regulator that ubiquitinates and degrades BnaTBCC1 to modulate drought tolerance and provided desirable germplasm for further breeding of drought tolerance in rapeseed.
干旱胁迫对大田作物构成持续威胁，严重限制了全球农业生产力。植物利用泛素依赖性降解作为一种关键的翻译后调控机制，以快速适应不断变化的环境条件。JUL1 是拟南芥中与干旱胁迫相关的 RING 型 E3 连接酶。在本研究中，我们探索了 BnaJUL1（甘蓝型油菜中 JUL1 的同源基因）的功能，并发现了一个参与耐旱性的新基因 BnaTBCC1。首先，我们利用 CRISPR-CRISPR 相关蛋白 9 系统的 BnaJUL1-cri 材料。其次，我们通过耐旱性测定和转录组分析证实了 BnaJUL1 调节耐旱性。然后，我们通过酵母文库筛选鉴定了一系列与 BnaJUL1 相互作用的蛋白质，包括 BnaTBCC1（微管结合因子 C 域蛋白）；其同源基因 TBCC1 敲低突变体（tbcc1-1）在拟南芥中表现出 ABA 敏感的萌发，我们随后证实了 BnaTBCC1 参与了拟南芥和油菜的耐旱性。最后，我们确定 BnaJUL1 可以泛素化和降解 BnaTBCC1 来调节耐旱性。因此，我们的研究揭示了 BnaJUL1 作为一种新的调节因子，通过泛素化和降解 BnaTBCC1 来调节耐旱性，并为油菜耐旱性的进一步育种提供了理想的种质资源。
