State Key Laboratory of Meat Quality Control and Cultured Meat Development, MOE, Jiangsu Synergetic Innovation Center of Meat Processing and Quality Control, College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Instituto de Agroquímica y Tecnología de Alimentos (CSIC), Avenue Agustín Escardino 7, Paterna, Valencia 46980, Spain.
Ultrason Sonochem. 2023 Dec;101:106697. doi: 10.1016/j.ultsonch.2023.106697. Epub 2023 Nov 18.
In this study, the effects of ultrasound-assisted enzymatic hydrolysis on the extraction of anti-inflammatory peptides from porcine bone collagen were investigated. The results showed that ultrasound treatment increased the content of α-helix while decreased β-chain and random coil, promoted generation of small molecular peptides. Ultrasound-assisted enzymatic hydrolysis improved the peptide content, enhanced ABTS radical scavenging and ferrous ion chelating ability than non-ultrasound group. At the ultrasonic power of 450 W (20 min), peptides possessed significant anti-inflammatory activity, where the releasing of interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) was all suppressed in lipopolysaccharide (LPS) induced RAW264.7 cells. After the analysis with LC-MS/MS, eight peptides with potential anti-inflammatory activities were selected by the PeptideRanker and molecular docking. In general, the ultrasound-assisted enzymatic hydrolysis was an effective strategy to extract the bioactive peptides from porcine bone, and the inflammatory regulation capacity of bone collagen sourced peptides was firstly demonstrated.
在这项研究中，研究了超声辅助酶解对猪骨胶原蛋白中抗炎肽提取的影响。结果表明，超声处理增加了α-螺旋的含量，同时降低了β-链和无规卷曲，促进了小分子肽的生成。超声辅助酶解提高了肽的含量，增强了 ABTS 自由基清除能力和亚铁离子螯合能力，优于非超声组。在超声功率为 450 W（20 min）时，肽具有显著的抗炎活性，其中脂多糖（LPS）诱导的 RAW264.7 细胞中白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的释放均受到抑制。通过 LC-MS/MS 分析，用 PeptideRanker 和分子对接筛选出了 8 种具有潜在抗炎活性的肽。总的来说，超声辅助酶解是一种从猪骨中提取生物活性肽的有效策略，首次证明了骨胶原来源肽的抗炎调节能力。
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