芳基烃受体 (AhR) 的激活介导了苯并 (a) 芘诱导的 HaCaT 细胞中水通道蛋白 3 和 Notch1 的过度表达。
The aryl hydrocarbon receptor (AhR) activation mediates benzo(a)pyrene-induced overexpression of AQP3 and Notch1 in HaCaT cells.
机构信息
Laboratorio de Toxicología Molecular, Centro de Investigación Aplicada en Ambiente y Salud (CIAAS), Coordinación para la Innovación y Aplicación de la Ciencia y la Tecnología (CIACYT), Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
Facultad de Medicina, Universidad Autónoma de San Luis Potosí, San Luis Potosí, Mexico.
出版信息
Environ Mol Mutagen. 2023 Oct-Nov;64(8-9):466-472. doi: 10.1002/em.22580. Epub 2023 Nov 30.
The aim of this study was twofold: (1) evaluate the effect of benzo[a]pyrene (BaP) on expression levels of AQP3 and Notch1 genes in HaCaT cells exposed "in vitro" and (2) investigate the possible biological role of assessed genes by bioinformatics methods. Cells were exposed to increasing concentrations of BaP (0.0-4.0 μM) for 1-4 days. After treatments, cell viability and expression levels of AhR, CYP1A1, AQP3, and Notch1 genes were evaluated. The possible biological role of assessed genes was evaluated using bioinformatics tools. Low cytotoxicity in HaCaT cells dosed with BaP was detected. A significant overexpression (p < .05) of CYP1A1, AQP3, and Notch1 was found in exposed HaCaT cells. The gene expression upregulation was dependent on AhR activation. The bioinformatics analysis showed that these genes were enriched in related cancer signaling pathways. The findings suggest that AQP3 and Notch1 are upregulated by AhR activation in HaCaT cells exposed to BaP.
本研究旨在评估苯并[a]芘（BaP）对体外暴露的 HaCaT 细胞中 AQP3 和 Notch1 基因表达水平的影响，并通过生物信息学方法探讨评估基因的可能生物学作用。将细胞暴露于不同浓度的 BaP（0.0-4.0 μM）中 1-4 天。处理后，评估 AhR、CYP1A1、AQP3 和 Notch1 基因的细胞活力和表达水平。使用生物信息学工具评估评估基因的可能生物学作用。用 BaP 处理的 HaCaT 细胞的细胞毒性低。在暴露的 HaCaT 细胞中，CYP1A1、AQP3 和 Notch1 的表达显著上调（p <.05）。基因表达的上调依赖于 AhR 的激活。生物信息学分析表明，这些基因在相关的癌症信号通路中富集。研究结果表明，AQP3 和 Notch1 在 BaP 暴露的 HaCaT 细胞中通过 AhR 激活而上调。
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