Isolation, purification and characterization of β-glucan from cereals - A review.

β-glucans are soluble fibers found in cereal compounds, including barley, oats etc., as an active component. They are used as a dietary fiber to treat cholesterol, diabetes and cardiovascular diseases. These polysaccharides are important because they can provide many therapeutic benefits related to their biological activity in human like inhibiting tumour growth, anti-inflammatory action, etc. All these activities were usually attached to their molecular weight, structure and degree of branching. The present manuscript reviews the background of β-glucan, its characterization techniques, the possible ways to extract β-glucan and mainly focuses on membrane-based purification techniques. The β-glucan separation methods using polymeric membranes, their operational characteristics, purification methods which may yield pure or crude β-glucan and structural analysis methods were also discussed. Future direction in research and development related to β-glucan recovery from cereal were also offered.