Vanharen Marion, Mahbeer Thomas, Léveillé Alexanne, Méthot Audrey, Samountry Phonsiri, Girard Denis
Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.
Laboratoire de recherche en inflammation et physiologie des granulocytes, Université du Québec, INRS-Centre Armand-Frappier Santé Biotechnologie, Laval, Québec, Canada.
Environ Toxicol Pharmacol. 2023 Nov;104:104319. doi: 10.1016/j.etap.2023.104319. Epub 2023 Nov 19.
Some differences exist between the male and female immune systems. Despite this, a sex-based analysis is not frequently performed in most studies. Knowing that inflammation is a common undesired effect observed resulting from nanoparticle (NP) exposure, we investigate here how gold NPs with a primary size of 20 (AuNP) and 70 nm (AuNP) will alter the biology of polymorphonuclear neutrophil cells (PMNs) isolated from men and women as well as their potential pro-inflammatory effect in vivo in male and female mice. We found that AuNP significantly delay apoptosis only in PMN isolated from men. The production of interleukin (IL)- 8 by PMNs was increased by both AuNPs regardless of sex although significance was only observed in AuNP-induced PMNs. Using the murine air pouch model of inflammation, AuNPs did not induce a neutrophilic infiltration regardless of sex. In conclusion, AuNPs could differently alter the biology of PMNs according to sex.
男性和女性的免疫系统存在一些差异。尽管如此，大多数研究中并不经常进行基于性别的分析。鉴于炎症是纳米颗粒（NP）暴露后常见的不良效应，我们在此研究了初始尺寸为20纳米（AuNP）和70纳米（AuNP）的金纳米颗粒如何改变从男性和女性分离出的多形核中性粒细胞（PMN）的生物学特性，以及它们在雄性和雌性小鼠体内的潜在促炎作用。我们发现，AuNP仅在从男性分离出的PMN中显著延迟细胞凋亡。无论性别如何，两种AuNP均会增加PMN产生白细胞介素（IL）-8的量，不过只有AuNP诱导的PMN表现出显著差异。使用小鼠气袋炎症模型，无论性别如何，AuNP均未诱导嗜中性粒细胞浸润。总之，AuNP可能会根据性别不同地改变PMN的生物学特性。
