Rosen M B, Crofton K M, Chernoff N
Toxicol Lett. 1986 Dec;34(2-3):223-9. doi: 10.1016/0378-4274(86)90214-6.
Pregnant Sprague-Dawley rats were exposed to either 3500 or 7000 mg/m3 p-xylene from days 7-16 of gestation. Dams were allowed to give birth, and litters were counted, weighed, and observed for external malformations on postnatal days (PD) 1 and 3. Litters were normalized to 8 pups (4 males and 4 females +/- 1) on PD4. On PD21 animals were weaned and littermates housed by sex. Body weights were recorded weekly until weaning and once every 2 weeks thereafter. Central nervous system (CNS) development was evaluated by acoustic startle response on PD13, 17, 21, and 63 as well as figure-8 maze activity on PD22 and 65. Maternal weight gain during the treatment period was significantly less in the high-dose group. No effects were seen on litter size or weight at birth or on PD3. There were no effects of xylene exposure on growth rate. There were no treatment-related effects on acoustic startle response or figure-8 maze activity. Thus, p-xylene as administered in this study does not appear to be a selective developmental toxicant in the rat.
将妊娠第7至16天的斯普拉格-道利孕鼠暴露于3500或7000毫克/立方米的对二甲苯环境中。让母鼠分娩，统计出生窝仔数、称重，并在出生后第1天和第3天观察仔鼠的外部畸形情况。在出生后第4天将窝仔数标准化为8只幼崽（4只雄性和4只雌性，±1只）。在出生后第21天给动物断奶，并按性别将同窝仔鼠饲养在一起。记录每周直至断奶时的体重，此后每两周记录一次体重。在出生后第13、17、21和63天通过听觉惊吓反应以及在出生后第22和65天通过8字形迷宫活动评估中枢神经系统（CNS）的发育情况。高剂量组在治疗期间的母鼠体重增加显著较少。在出生时或出生后第3天，未观察到对窝仔数或体重有影响。二甲苯暴露对生长速率没有影响。对听觉惊吓反应或8字形迷宫活动没有与治疗相关的影响。因此，本研究中所施用的对二甲苯在大鼠中似乎不是一种选择性发育毒物。
