Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil.
Nanotechnology National Laboratory for Agriculture (LNNA), Embrapa Instrumentation, 13560-970 São Carlos, SP, Brazil; PPGQ, Department of Chemistry, Center for Exact Sciences and Technology, Federal University of São Carlos (UFSCar), 13565-905 São Carlos, SP, Brazil.
Carbohydr Polym. 2024 Jan 15;324:121494. doi: 10.1016/j.carbpol.2023.121494. Epub 2023 Oct 14.
The growing demand for opto-electronic devices within an automated landscape has opened up new opportunities for harnessing sustainable cellulose materials for sensors technology. Cellulose, a versatile material, enables its combination with other materials, but in most of these applications, cellulose is typically employed as support or substrate, while its inherent autofluorescence remains largely underexplored for sensors. In light of this context, this study delves into the autofluorescence characteristics of pristine cellulose nanocrystals extracted from wood via enzymatic route for optical sensors tailored to detect tannins. By fine-tuning the experimental setup, photoluminescence (PL) emission bands were scrutinized across three distinct spectral regions, namely 300-400 nm, 400-500 nm and 550-700 nm. The proposed mechanism reveals the occurrence of dynamic fluorescence quenching, which enabled the selective monitoring of tannins in red wines across a dynamic range spanning from 10 to 1060 μg mL. This sensing platform provided a limit of detection (LoD) of 6.1 μg mL. Notably, the sensing platform's efficacy was validated with remarkable recovery rates of 99.7 % and 95.3 % when subjected to testing with cabernet sauvignon and tannat wines. These findings emphasize the sensing platform's potential for monitoring tannic acids in beverages and food products.
在自动化环境中，对光电子器件的需求不断增长，为利用可持续的纤维素材料开拓了新的机会，以用于传感器技术。纤维素是一种多功能材料，可与其他材料结合使用，但在大多数这些应用中，纤维素通常用作支撑或基底，而其固有的自发荧光在传感器应用中仍在很大程度上未得到充分探索。有鉴于此，本研究深入探讨了通过酶法从木材中提取的原始纤维素纳米晶体的自发荧光特性，旨在为检测单宁的光学传感器开发。通过微调实验设置，在三个不同的光谱区域（300-400nm、400-500nm 和 550-700nm）仔细研究了光致发光（PL）发射带。所提出的机制揭示了动态荧光猝灭的发生，这使得能够在 10 至 1060μgmL 的动态范围内选择性监测红酒中的单宁。该传感平台的检测限（LoD）为 6.1μgmL。值得注意的是，当用赤霞珠和 tannat 葡萄酒进行测试时，该传感平台的功效表现出了显著的回收率，分别为 99.7%和 95.3%。这些发现强调了该传感平台在监测饮料和食品中鞣酸的潜力。
