Department of Animal Science and Technology, Chung-Ang University, Anseong 17546, Korea.
Division of Animal Science, Division of Applied Life Science (BK21 Four), Institute of Agriculture & Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea.
Food Res Int. 2023 Dec;174(Pt 1):113617. doi: 10.1016/j.foodres.2023.113617. Epub 2023 Oct 24.
Fetal bovine serum (FBS) is an extremely important culture growth supplement, accounting for approximately 60 % of cell-culture-media costs; therefore, lowering FBS-acquisition costs for the industrialization of cultured meat is imperative. This study attempted to produce an FBS substitute using discarded livestock by-products, with particular focus on formulating a product with a composition similar to that of FBS to improve effectiveness. However, to date, no study has precisely analyzed the commercial components of FBS, and this study is the first to compare the chemical composition of FBS and commercially available horse serum purchased from the United States or Europe with that of FBS substitutes developed by our team. This study analyzed the chemical composition of the FBS products purchased by our team over the past 3 years via blood, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and independent composition analyses. While the composition and quality of commercial FBS products are known to vary, the FBS composition of our purchased products was relatively uniform regardless of company, brand, or country of origin. In contrast, FBS substitutes obtained from three major livestock species (cattle, pig, and chicken) clearly exhibited differences in composition, a phenomenon that was also observed upon comparing with FBS as well as among different species. Therefore, to replace commercial FBS entirely, the production of a proportionately effective substitute product comprising an equal or similar composition is required, and the results of this study can be a steppingstone to achieving this. In addition, FBS substitutes manufactured using inexpensive slaughter by-products as raw materials are expected to ultimately reduce the unit cost of cultured meat production.
胎牛血清（FBS）是一种极其重要的培养生长补充剂，约占细胞培养介质成本的 60%；因此，降低用于培养肉产业化的 FBS 采购成本是当务之急。本研究试图使用废弃的牲畜副产品来生产 FBS 替代品，特别注重制定一种与 FBS 组成相似的产品，以提高其效果。然而，迄今为止，还没有研究精确分析 FBS 的商业成分，本研究首次比较了 FBS 和我们团队开发的 FBS 替代品的化学成分，以及从美国或欧洲购买的市售马血清的化学成分。本研究通过血液、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和独立成分分析，分析了我们团队在过去 3 年购买的 FBS 产品的化学成分。虽然商业 FBS 产品的组成和质量众所周知会有所不同，但我们购买的产品的 FBS 组成相对均匀，无论公司、品牌或原产国如何。相比之下，从三大牲畜物种（牛、猪和鸡）获得的 FBS 替代品在组成上明显存在差异，这一现象在与 FBS 以及不同物种之间进行比较时也观察到了。因此，要完全替代商业 FBS，需要生产一种组成相同或相似、具有同等效果的替代品，本研究的结果可以为此提供一个起点。此外，使用廉价的屠宰副产品作为原材料制造的 FBS 替代品有望最终降低培养肉生产的单位成本。
