Vester Susan K, Davies Anna M, Beavil Rebecca L, Sandhar Balraj S, Beavil Andrew J, Gould Hannah J, Sutton Brian J, McDonnell James M
Randall Centre for Cell and Molecular Biophysics, King's College London, New Hunt's House, London SE1 1UL, UK.
Antibodies (Basel). 2023 Nov 16;12(4):75. doi: 10.3390/antib12040075.
We have previously produced a toolkit of antibodies, comprising recombinant human antibodies of all but one of the human isotypes, directed against the polcalcin family antigen Phl p 7. In this work, we complete the toolkit of human antibody isotypes with the IgD version of the anti-Phl p 7 monoclonal antibody. We also raised a set of nanobodies against the IgD anti-Phl p 7 antibody and identify and characterize one paratope-specific nanobody. This nanobody also binds to the IgE isotype of this antibody, which shares the same idiotype, and orthosterically inhibits the interaction with Phl p 7. The 2.1 Å resolution X-ray crystal structure of the nanobody in complex with the IgD Fab is described.
我们之前制备了一套抗体工具，其中包含除一种人类同种型外的所有重组人抗体，这些抗体针对多聚钙蛋白家族抗原Phl p 7。在这项工作中，我们用抗Phl p 7单克隆抗体的IgD版本完善了人类抗体同种型工具。我们还制备了一组针对IgD抗Phl p 7抗体的纳米抗体，并鉴定和表征了一种互补决定区特异性纳米抗体。这种纳米抗体也能结合该抗体的IgE同种型，它们具有相同的独特型，并且能在正位上抑制与Phl p 7的相互作用。本文描述了该纳米抗体与IgD Fab复合物的2.1 Å分辨率X射线晶体结构。
