Centro de Biotecnología y Genómica de Plantas UPM-INIA/CSIC and E.T.S. Ingeniería Agronómica, Alimentaria y de Biosistemas, Universidad Politécnica de Madrid, Madrid, Spain.
Plant Physiology, Pharmaceutical and Health Sciences Department, Faculty of Pharmacy, Universidad San Pablo-CEU, CEU-Universities, Boadilla del Monte, Madrid, Spain.
Methods Mol Biol. 2024;2724:181-192. doi: 10.1007/978-1-0716-3485-1_13.
More than 25% of all known plant viruses are transmitted through seeds, which makes this mode of dispersal of great importance for plant virus epidemics. Virus detection in seed stocks remains the most frequent approach for seed health testing, but current methods are not always standardized and/or do not allow analyzing large numbers of seeds. Here, we describe a high-throughput method to quantify plant virus seed transmission rate based on classical grow-out tests, which can be applied to widely different viruses and host species.
超过 25%的已知植物病毒通过种子传播，这使得这种传播方式对植物病毒病的流行非常重要。在种子库存中检测病毒仍然是种子健康测试最常用的方法，但目前的方法并不总是标准化和/或不允许分析大量的种子。在这里，我们描述了一种基于经典生长试验的高通量方法来量化植物病毒种子传播率，该方法可应用于广泛不同的病毒和宿主物种。
