Weeks J R
Prostaglandins. 1979 Apr;17(4):495-9. doi: 10.1016/0090-6980(79)90002-9.
Prostacyclin is short acting and chemically unstable. To study sustained effects in an intact animal, prolonged intravenous infusion may be required. The compound has adequate stability for 24 hr in pH 10.0 carbonate buffer at 0 degrees. A "displacement syringe" is described wherein the prostacyclin solution is stored in a rubber bag inside the barrel of a 5 ml syringe. This device is placed in an ice bath. A syringe pump drives water into the barrel displacing an equal volume of solution out of the bag. Chronic venous cannules, saddles, and flow-through swivels are used as for drug self-administration studies. A simple, inexpensive rack for use with conventional individual hanging cages is also described.
前列环素作用时间短且化学性质不稳定。为了在完整动物中研究其持续效应，可能需要长时间静脉输注。该化合物在0摄氏度的pH 10.0碳酸盐缓冲液中24小时具有足够的稳定性。描述了一种“置换注射器”，其中前列环素溶液储存在5毫升注射器筒内的橡胶袋中。该装置置于冰浴中。注射泵将水注入筒内，将等量的溶液从袋中置换出来。慢性静脉插管、鞍座和流通式旋转接头用于药物自我给药研究。还描述了一种与传统单个悬挂笼配合使用的简单、廉价的架子。
