Yazdanpanah Somayeh, Rahbarmah Maryam, Motamedi Marjan, Khodadadi Hossein
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran..
Diagn Microbiol Infect Dis. 2024 Feb;108(2):116118. doi: 10.1016/j.diagmicrobio.2023.116118. Epub 2023 Oct 27.
(1-3)-Beta-D Glucan (BDG) detection has shown to be a highly effective tool to diagnose invasive fungal infections. Therefore, this study aimed to compare clinical characteristics of the Fungitell (FA) and Dynamiker Fungus (1-3)-β-D-Glucan assay (DFA) for the diagnosis of candidemia. Using DFA and FA, the BDG levels of 57 serum samples from case and control groups were determined. The kappa coefficient (κ) and Spearman's rank correlation (r) were used to examine the consistency of assays on a quantitative and qualitative level, respectively. The sensitivity, specificity, and accuracy were 94.6 %, 65.0 %, and 87.7% for DFA, and 94.6 %, 75.0 %, and 89.4 % for FA, respectively. The performance of the DFA for the diagnosis of candidemia was highly consistent with that of the FA, both quantitatively (r: 0.9) and qualitatively (kappa = 0.78). Collectively, the DFA assay performed excellently in comparison to the FA for the diagnosis of candidemia.
（1-3）-β-D葡聚糖（BDG）检测已被证明是诊断侵袭性真菌感染的一种高效工具。因此，本研究旨在比较用于诊断念珠菌血症的真菌检测试剂盒（FA）和动态真菌（1-3）-β-D-葡聚糖检测法（DFA）的临床特征。使用DFA和FA测定了病例组和对照组57份血清样本的BDG水平。kappa系数（κ）和Spearman等级相关性（r）分别用于在定量和定性水平上检验检测方法的一致性。DFA的灵敏度、特异性和准确性分别为94.6%、65.0%和87.7%，FA的分别为94.6%、75.0%和89.4%。DFA用于诊断念珠菌血症的性能在定量（r：0.9）和定性（kappa = 0.78）方面都与FA高度一致。总体而言，与FA相比，DFA检测法在诊断念珠菌血症方面表现出色。
