Waddell Lindsey A, Wu Zhiguang, Sauter Kristin A, Hope Jayne C, Hume David A
The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
The Roslin Institute and R(D)SVS, University of Edinburgh, Easter Bush, Midlothian EH25 9RG, UK.
Vet Immunol Immunopathol. 2023 Dec;266:110681. doi: 10.1016/j.vetimm.2023.110681. Epub 2023 Nov 18.
Macrophage colony-stimulating factor (CSF1) controls the proliferation and differentiation of cells of the mononuclear phagocyte system through binding to the receptor CSF1R. The expression and function of CSF1 has been well-studied in rodents and humans, but knowledge is lacking in other veterinary species. The development of a novel mouse anti-porcine CSF1 monoclonal antibody (mAb) facilitates the characterisation of this growth factor in pigs. Cell surface expression of CSF1 was confirmed on differentiated macrophage populations derived from blood and bone marrow monocytes, and on lung resident macrophages, the first species for this to be confirmed. However, monocytes isolated from blood and bone marrow lacked CSF1 expression. This species-specific mAb delivers the opportunity to further understanding of porcine myeloid cell biology. This is not only vital for the role of pigs as a model for human health, but also as a veterinary species of significant economic and agricultural importance.
巨噬细胞集落刺激因子（CSF1）通过与受体CSF1R结合来控制单核吞噬细胞系统细胞的增殖和分化。CSF1的表达和功能在啮齿动物和人类中已得到充分研究，但在其他兽医物种中仍缺乏相关知识。一种新型小鼠抗猪CSF1单克隆抗体（mAb）的开发有助于在猪中对这种生长因子进行表征。在源自血液和骨髓单核细胞的分化巨噬细胞群体以及肺驻留巨噬细胞（首个被证实的物种）上证实了CSF1的细胞表面表达。然而，从血液和骨髓中分离出的单核细胞缺乏CSF1表达。这种物种特异性单克隆抗体为进一步了解猪髓细胞生物学提供了机会。这不仅对猪作为人类健康模型的作用至关重要，而且对作为具有重要经济和农业意义的兽医物种也至关重要。
