MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, China; College of Life and Environmental Sciences, Minzu University of China, Beijing 100081, China; Key Laboratory of Mass Spectrometry Imaging and Metabolomics (State Ethnic Affairs Commission), Centre for Imaging & Systems Biology, Minzu University of China, Beijing, 100081, China.
MOE Key Laboratory of Resources and Environmental Systems Optimization, College of Environmental Science and Engineering, North China Electric Power University, Beijing, 102206, China.
Environ Res. 2024 Feb 1;242:117717. doi: 10.1016/j.envres.2023.117717. Epub 2023 Nov 21.
Understanding the behavior and potential toxicity of copper nanoparticles (nano-Cu) in the aquatic environment is a primary way to assess their environmental risks. In this study, RNA-seq was performed on three different tissues (gills, intestines, and muscles) of zebrafish exposed to nano-Cu, to explore the potential toxic mechanism of nano-Cu on zebrafish. The results indicated that the toxic mechanism of nano-Cu on zebrafish was tissue-specific. Nano-Cu enables the CB1 receptor of the presynaptic membrane of gill cells to affect short-term synaptic plasticity or long-term synaptic changes (ECB-LTD) through DSI and DSE, causing dysfunction of intercellular signal transmission. Imbalance of de novo synthesis of UMP in intestinal cells and its transformation to UDP, UTP, uridine, and uracil, resulted in many functions involved in the pyrimidine metabolic pathway being blocked. Meanwhile, the toxicity of nano-Cu caused abnormal expression of RAD51 gene in muscle cells, which affects the repair of damaged DNA through Fanconi anemia and homologous recombination pathway, thus causing cell cycle disorder. These results provide insights for us to better understand the differences in toxicity of nano-Cu on zebrafish tissues and are helpful for a comprehensive assessment of nano-Cu's effects on aquatic organisms.
了解铜纳米粒子（nano-Cu）在水生环境中的行为和潜在毒性是评估其环境风险的主要方法。在这项研究中，对暴露于 nano-Cu 的斑马鱼的三个不同组织（鳃、肠和肌肉）进行了 RNA 测序，以探讨 nano-Cu 对斑马鱼的潜在毒性机制。结果表明，nano-Cu 对斑马鱼的毒性机制具有组织特异性。nano-Cu 通过 DSI 和 DSE 影响鳃细胞的突触前膜的 CB1 受体，从而影响短期突触可塑性或长期突触变化（ECB-LTD），导致细胞间信号转导功能障碍。肠道细胞中 UMP 的从头合成失衡及其向 UDP、UTP、尿嘧啶和尿苷的转化，导致嘧啶代谢途径中许多涉及的功能受阻。同时，nano-Cu 的毒性导致肌肉细胞中 RAD51 基因的异常表达，通过范可尼贫血和同源重组途径影响受损 DNA 的修复，从而导致细胞周期紊乱。这些结果为我们更好地了解 nano-Cu 对斑马鱼组织毒性的差异提供了深入的认识，并有助于全面评估 nano-Cu 对水生生物的影响。
