UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
UCD School of Biomolecular and Biomedical Science, University College Dublin, Belfield, Dublin 4, Ireland.
Fungal Biol. 2023 Oct-Nov;127(10-11):1384-1388. doi: 10.1016/j.funbio.2023.10.001. Epub 2023 Oct 14.
The filamentous fungus Cunninghamella echinulata is a model of mammalian xenobiotic metabolism. Under certain conditions it grows as a biofilm, which is a natural form of immobilisation and enables the fungus to catalyse repeated biotransformations. Putative signalling molecules produced by other Cunninghamella spp., such as 3-hydroxytyrosol and tyrosol, do not affect the biofilm growth of C. echinulata, suggesting that it employs a different molecule to regulate biofilm growth. In this paper we report that 2-phenylethanol is produced in higher concentrations in planktonic cultures of C. echinulata than when the fungus is grown as a biofilm. We demonstrate that exogenously added 2-phenylethanol inhibits biofilm growth of C. echinulata but has no effect on planktonic growth. Furthermore, we show that addition of 2-phenylethanol to established C. echinulata biofilm causes detachment. Therefore, we conclude that this molecule is produced by the fungus to regulate biofilm growth.
丝状真菌卷枝毛霉是哺乳动物异生物质代谢的模型。在某些条件下，它生长为生物膜，这是一种自然的固定化形式，使真菌能够催化重复的生物转化。其他卷枝毛霉属产生的假定信号分子，如 3-羟基酪醇和酪醇，不会影响 C. echinulata 的生物膜生长，这表明它使用不同的分子来调节生物膜生长。在本文中，我们报告说，与真菌生长为生物膜相比，C. echinulata 的浮游培养物中产生的 2-苯乙醇浓度更高。我们证明，外源添加的 2-苯乙醇抑制 C. echinulata 的生物膜生长，但对浮游生长没有影响。此外，我们还表明，向已建立的 C. echinulata 生物膜中添加 2-苯乙醇会导致其脱落。因此，我们得出结论，这种分子是由真菌产生的，以调节生物膜的生长。
