Clinical Pharmacology Research Laboratory, Yokohama University of Pharmacy, Yokohama, Kanagawa, Japan.
Planning and Product Development Division, Nippon Sigmax Co., Ltd., Shinjuku-ku, Tokyo, Japan.
J Ultrasound Med. 2024 Feb;43(2):385-395. doi: 10.1002/jum.16371. Epub 2023 Nov 22.
The intermittent injection of teriparatide, a recombinant fragment of human parathyroid hormone (PTH 1-34), activates anabolic activity on bone turnover. However, the PTH administration period is limited to 2 years. Thus, sequential therapy after discontinuation of PTH is required. Low-intensity pulsed ultrasound (LIPUS) has been widely used for bone fracture healing. In this study, we examined the effects of LIPUS on bone mass after PTH withdrawal in ovariectomized (OVX) model mice. The LIPUS-non-irradiated femoral trabecular bone mineral density (BMD) in the treated after PTH withdrawal was significantly decreased. Meanwhile, the femoral BMD in the OVX + PTH-LIPUS group was remarkably higher than that of the OVX group. Additionally, mRNA expression of Runx2, Osterix, Col1a1, and ALP increased significantly following LIPUS irradiation after PTH withdrawal. These results suggest that LIPUS protected against femoral trabecular BMD loss and up-regulated the osteogenic factors following PTH withdrawal in OVX mice.
特立帕肽（甲状旁腺激素 1-34 的重组片段）的间歇性注射可激活骨转换的合成代谢活性。然而，PTH 的给药期限制在 2 年以内。因此，需要在 PTH 停药后进行序贯治疗。低强度脉冲超声（LIPUS）已广泛用于骨骨折愈合。在这项研究中，我们研究了 LIPUS 在去卵巢（OVX）模型小鼠 PTH 停药后对骨量的影响。与 PTH 停药后的未照射股骨小梁骨密度（BMD）相比，LIPUS 照射后的股骨 BMD 明显降低。同时，OVX+PTH-LIPUS 组的股骨 BMD 明显高于 OVX 组。此外，PTH 停药后 LIPUS 照射可显著增加 Runx2、Osterix、Col1a1 和 ALP 的 mRNA 表达。这些结果表明，LIPUS 可防止 OVX 小鼠股骨小梁 BMD 丢失，并上调 PTH 停药后的成骨因子。
