Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, India.
Department of Biosciences and Bioengineering, Indian Institute of Technology Roorkee, India.
Plant Physiol Biochem. 2023 Dec;205:108195. doi: 10.1016/j.plaphy.2023.108195. Epub 2023 Nov 19.
Iron and zinc deficiencies are the most prevalent cause of global hidden hunger. Rice, being one of the most consumed crops worldwide, is suitable to target for Fe and Zn biofortification. In present study, we generated rice transgenic lines to meet the recommended dietary requirement of iron and zinc through endosperm specific expression of dicot (kidney bean) and monocot (pearl millet) Ferritins along with constitutive expression of rice nicotianamine synthase 2 (OsNAS2) gene. Visualization through perls' prussian staining and quantification by ICP-MS showed significant improvement in grain iron content in all the transgenic lines. The transgenic lines expressing any of the three selected gene combinations (PvFerrtin-OsNAS2, feedPgFerrtin-OsNAS2 and foodPgFerritin-OsNAS2), showed the potential to surpass the 30% of the estimated average requirement (13 μg/g Fe and 28 μg/g Zn) proposed for rice in HarvestPlus breeding program. Though the expression of PvFerritin along with OsNAS2 gene in IET10364 (indica) variety showed the best result, providing up to 4.2- and 3.5-fold increase in iron (30.56 μg/g) and zinc (60.1 μg/g) content, respectively; in polished grains compared to non-transgenic control. Thus, the lines developed in our study can be used for further breeding purpose to enhance the iron and zinc content in commercial rice varieties.
铁和锌缺乏是全球隐性饥饿最普遍的原因。水稻是全球消费最多的作物之一，适合作为铁和锌生物强化的目标。在本研究中，我们通过胚乳特异性表达双子叶（菜豆）和单子叶（珍珠粟）铁蛋白以及组成型表达水稻尼克酸合成酶 2（OsNAS2）基因，生成了水稻转基因系，以满足铁和锌的推荐膳食需求。通过 Perl's 普鲁士染色可视化和 ICP-MS 定量显示，所有转基因系的籽粒铁含量都有显著提高。在表达三种选定基因组合（PvFerrtin-OsNAS2、feedPgFerrtin-OsNAS2 和 foodPgFerritin-OsNAS2）的转基因系中，都有可能超过 HarvestPlus 育种计划中为水稻提出的 30%的估计平均需求量（13μg/g Fe 和 28μg/g Zn）。尽管在 indica 品种 IET10364 中表达 PvFerritin 与 OsNAS2 基因的效果最好，与非转基因对照相比，在糙米中铁（30.56μg/g）和锌（60.1μg/g）的含量分别增加了 4.2 倍和 3.5 倍；但是，我们在研究中开发的系可以用于进一步的育种目的，以提高商业水稻品种的铁和锌含量。
