[大肠杆菌中的鸟苷酸环化酶。I. 酶的纯化及抑制剂的证据]
[Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor].
作者信息
Macchia V, Rocino A, Gulletta E, Consiglio E, Varrone S
出版信息
C R Seances Soc Biol Fil. 1978;172(5):860-7.
PMID:37997
Abstract
Guanylate cyclase activity is present in crude E. coli extract. Guanylate cyclase has been purified 3500 fold from this extract, through ammonium sulfate fractionation, DEAE-cellulose chromatography. Sephadex G-75 gel-filtration and polyacrylamide gel preparative microelectrophoresis. During the purification a guanylate cyclase inhibitor has been separated.
摘要
大肠杆菌粗提物中存在鸟苷酸环化酶活性。通过硫酸铵分级沉淀、DEAE-纤维素色谱、Sephadex G-75凝胶过滤和聚丙烯酰胺凝胶制备型微电泳，已从该提取物中纯化出3500倍的鸟苷酸环化酶。在纯化过程中分离出了一种鸟苷酸环化酶抑制剂。
相似文献
1
[Guanylate cyclase in Escherichia coli. I. Purification of the enzyme and evidence for an inhibitor].[大肠杆菌中的鸟苷酸环化酶。I. 酶的纯化及抑制剂的证据]
C R Seances Soc Biol Fil. 1978;172(5):860-7.
2
[Guanyl cyclase activity in the EF-T elongation factor of Escherichia coli].[大肠杆菌EF-T延伸因子中的鸟苷酸环化酶活性]
C R Seances Soc Biol Fil. 1977;171(3):516-25.
3
Partial purification and characterization of particulate guanylate cyclase from rat liver after solubilization with trypsin.用胰蛋白酶溶解后大鼠肝脏颗粒型鸟苷酸环化酶的部分纯化及特性分析
J Cyclic Nucleotide Res. 1982;8(6):359-70.
4
[Guanylate cyclase in E. coli. III. Purification and possible physiological role of GTPase].[大肠杆菌中的鸟苷酸环化酶。III. GTP酶的纯化及可能的生理作用]
C R Seances Soc Biol Fil. 1978;172(6):1079-84.
5
Guanylate cyclase in Escherichia coli. Purification and properties.大肠杆菌中的鸟苷酸环化酶。纯化及性质
J Biol Chem. 1975 Aug 25;250(16):6214-7.
6
[Purification and properties of phenylalanyl-tRNA-synthetase from Escherichia coli MRE-600].[来自大肠杆菌MRE - 600的苯丙氨酰 - tRNA合成酶的纯化及性质]
Prikl Biokhim Mikrobiol. 1984 Mar-Apr;20(2):208-16.
7
Purification of soluble guanylate cyclase enzyme from human platelets.
Biochem Int. 1989 Nov;19(5):1039-44.
8
[An endogenous regulator of the guanylate cyclase activity of human platelets].[人血小板鸟苷酸环化酶活性的一种内源性调节剂]
Biokhimiia. 1990 Oct;55(10):1812-8.
9
[Guanyl cyclase in Escherichia coli. II. Identification and characteristics on the enzyme inhibitor].[大肠杆菌中的鸟苷酸环化酶。II. 酶抑制剂的鉴定及特性]
C R Seances Soc Biol Fil. 1978;172(5):868-73.
10
[Guanylate kinase from bovine retina: isolation, primary structure, and expression in E. coli].
Bioorg Khim. 1994 Apr;20(4):367-81.
Zotero 插件