Department of Gastroenterology, Pingyang Hospital Affiliated to Wenzhou Medical University, Zhejiang, China.
Kaohsiung J Med Sci. 2024 Feb;40(2):131-138. doi: 10.1002/kjm2.12784. Epub 2023 Nov 23.
This study aimed to investigate the expression levels of tissue and serum miR-149-3p and miR-149-5p in hospitalized patients with inflammatory bowel disease (IBD). A total of 35 ulcerative colitis (UC) patients, 12 Crohn's disease (CD) patients, and 25 healthy controls were included in the study. The miRNAs expressions were measured in tissue and serum samples using quantitative real-time polymerase chain reaction (qRT-PCR). Inflammatory biomarkers were measured, including serum albumin, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), and fecal calprotectin. MiR-149-3p and miR-149-5p were significantly decreased in the inflamed areas of both CD and UC patients compared to tissue controls, which was consistent with decreased serum levels in IBD patients compared to healthy controls. When distinguishing UC patients from healthy controls, serum miR-149-3p showed 74% sensitivity and 96% specificity, while serum miR-149-5p exhibited 63% sensitivity and 96% specificity. In the CD versus healthy control comparison, miR-149-3p achieved 100% sensitivity and 96% specificity, while miR-149-5p demonstrated 92% sensitivity and 96% specificity. In the UC versus CD comparison, miR-149-5p showed 75% sensitivity and 77% specificity, while miR-149-3p displayed 67% sensitivity and 80% specificity. Significant correlations were identified between the tissue and serum expression of miR-149-3p/5p and disease activity scores, as well as inflammatory biomarkers in both CD and UC patients. Decreased expression of miR-149-3p and miR-149-5p is associated with disease activity in IBD patients. These miRNAs demonstrate diagnostic potential and may serve as biomarkers for monitoring disease activity in IBD.
本研究旨在探讨住院炎症性肠病（IBD）患者组织和血清 miR-149-3p 和 miR-149-5p 的表达水平。研究纳入了 35 例溃疡性结肠炎（UC）患者、12 例克罗恩病（CD）患者和 25 名健康对照者。采用实时定量聚合酶链反应（qRT-PCR）检测组织和血清样本中的 miRNA 表达。测量了炎症生物标志物，包括血清白蛋白、红细胞沉降率（ESR）、C 反应蛋白（CRP）、肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）和粪便钙卫蛋白。与组织对照相比，CD 和 UC 患者的炎症区域中 miR-149-3p 和 miR-149-5p 显著降低，这与 IBD 患者的血清水平低于健康对照者一致。在将 UC 患者与健康对照者区分时，血清 miR-149-3p 表现出 74%的灵敏度和 96%的特异性，而血清 miR-149-5p 则显示出 63%的灵敏度和 96%的特异性。在 CD 与健康对照者的比较中，miR-149-3p 达到 100%的灵敏度和 96%的特异性，而 miR-149-5p 则表现出 92%的灵敏度和 96%的特异性。在 UC 与 CD 的比较中，miR-149-5p 显示出 75%的灵敏度和 77%的特异性，而 miR-149-3p 则显示出 67%的灵敏度和 80%的特异性。组织和血清中 miR-149-3p/5p 的表达与 CD 和 UC 患者的疾病活动评分以及炎症生物标志物之间存在显著相关性。miR-149-3p 和 miR-149-5p 的表达降低与 IBD 患者的疾病活动相关。这些 miRNA 具有诊断潜力，可能作为监测 IBD 疾病活动的生物标志物。
