Zheng Yao, Addotey Tracy Naa Adoley, Chen Jiazhang, Xu Gangchun
Key Laboratory of Integrated Rice-Fish Farming Ecology, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center (FFRC), Chinese Academy of Fishery Sciences (CAFS), No. 9 Shanshui East Rd., Wuxi 214081, China.
Wuxi Fishery College, Nanjing Agricultural University, No. 9 Shanshui East Rd., Wuxi 214081, China.
Biology (Basel). 2023 Nov 14;12(11):1430. doi: 10.3390/biology12111430.
Recent studies have revealed a significant presence of microplastics (MPs) in freshwater ecosystems, raising concerns about their potential negative impacts on the growth and development of freshwater organisms. The present study was conducted to examine the effects of chronic sub-lethal doses of polystyrene microsphere MPs on the oxidative status (ROS, SOD) and the immune response (IL-1ß, TNF-α) of genetically improved farmed tilapia (a kind of tilapia hereafter referred to as GIFT). GIFT juveniles (5.1 ± 0.2 g) were exposed to different concentrations of substances. The experimental groups were as follows: group A (control, no exposure), group B (exposed to a concentration of 75 nm), group C (exposed to a concentration of 7.5 μm), group D (exposed to a concentration of 750 μm), group E (exposed to a combination of 75 nm, 7.5 μm, and 750 μm), and group F (exposed to a combination of 75 nm and ). The ROS contents in the brain and gills were significantly decreased in group F, while a significant increase was observed in group D following a 14-day exposure. SOD activities in the intestine showed an elevation in group F, as did those in the brain and gills in group D, while the SOD levels in the gills generally decreased over time in groups B and F. Notably, the highest ROS and SOD were observed in the brain of group D, whereas the lowest were in the intestines at the same concentration. The activity of IL-1β in the liver was significantly up-regulated in all of the exposure groups. IL-1β was significantly up-regulated in the brain of group B and in the gills of group D. Similarly, TNF-α was significantly up-regulated in the brain of groups B/D/E, in the liver of groups B/C/D, in the intestine of group B, and in the gills of group D. Notably, the highest levels of IL-1β and TNF-α activities were recorded in the brain, while the lowest were recorded in the intestine of group D. Overall, this study revealed that GIFT's immune response and antioxidant system can be affected by MPs.
最近的研究表明，淡水生态系统中存在大量微塑料（MPs），这引发了人们对其对淡水生物生长和发育潜在负面影响的担忧。本研究旨在探讨慢性亚致死剂量的聚苯乙烯微球MPs对遗传改良养殖罗非鱼（以下简称GIFT罗非鱼）氧化状态（ROS、SOD）和免疫反应（IL-1ß、TNF-α）的影响。GIFT幼鱼（5.1±0.2克）暴露于不同浓度的物质中。实验组如下：A组（对照组，未暴露）、B组（暴露于75纳米浓度）、C组（暴露于7.5微米浓度）、D组（暴露于750微米浓度）、E组（暴露于75纳米、7.5微米和750微米的组合）和F组（暴露于75纳米和……的组合）。暴露14天后，F组大脑和鳃中的ROS含量显著降低，而D组则显著增加。F组肠道中的SOD活性升高，D组大脑和鳃中的SOD活性也升高，而B组和F组鳃中的SOD水平通常随时间下降。值得注意的是，D组大脑中观察到的ROS和SOD最高，而在相同浓度下肠道中最低。所有暴露组肝脏中IL-1β的活性均显著上调。B组大脑和D组鳃中IL-1β显著上调。同样，B/D/E组大脑、B/C/D组肝脏、B组肠道和D组鳃中TNF-α显著上调。值得注意的是，IL-1β和TNF-α活性的最高水平记录在大脑中，而最低水平记录在D组的肠道中。总体而言，本研究表明GIFT罗非鱼的免疫反应和抗氧化系统会受到MPs的影响。
